Unraveling the Molecular Mechanisms of XRCC1 Gene SNPs in Thyroid Cancer Pathogenesis
Keywords:
XRCCI, SNPs, DNA, Genotype, Thyroid CancerAbstract
This study aims to find the association of polymorphism Arg194Trp (rs1799782) with thyroid cancer in Southern Punjab. For this purpose, 60 samples of cases and 60 samples of controls were collected from the Multan Institute of Nuclear Medicine and Radiotherapy (MINAR) along with associated data. Factors included in this study were gender, age, smoking status, alcohol consumption, family history of cancer, family history of thyroid cancer, diet, exposure to radiation in childhood, and diabetes. Clinicopathological characters included in the study were types of thyroid cancer and grade of tumor. In the case of women, the number of pregnancies, use of oral contraceptives, and menstruation history were included. DNA extraction was done by using the salt extraction method and for amplification of DNA tetra ARMS PCR was done. The frequency of three genotypes CC, TT, and CT of Arg194Trp (rs1799782) was found to be 49, 3, and 8 in patients while it was 43, 7, and 10 in controls respectively. The allelic frequency of C and T was 0.8 and 0.2 in controls respectively. While in patients’ allelic frequency of C and T was 0.9 and 0.1 respectively. For statistical analysis, SPSS version 20 was used. After statistical analysis, Arg194Trp polymorphism was not found to be associated with thyroid cancer. However, the association of this cancer was found with gender, family history of cancer, diet, oral contraceptives, and menstruation history.
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