Phytochemical screening of oil of Lactuca sativa seeds and determination of important bioactive and physicochemical aspects.

Authors

  • Dr.Ijaz Ali Assistant Professor Department of Pharmacognosy, Faculty of Pharmaceutical Sciences Government College University Faisalabad, Pakistan Author
  • Dr. Sohaib Iqbal Lecturer, Department of Pharmacy, Lyallpur Institute of Advanced Studies (LIAS), College of Pharmacy Faisalabad , Pakistan. Author
  • Dr. Muhammad Bilal Lecturer, Department of Pharmacy, Lyallpur Institute of Advance Sciences Faisalabad, Pakistan. Author
  • Dr. Muhammad Qazaf Lecturer, Department of Pharmacy, Lyallpur Institute of Advance Sciences Faisalabad, Pakistan. Author
  • Dr.Muhammad Muddassar Maqsood Lecturer, Department of Pharmacy, Lyallpur Institute of Advanced Studies Faisalabad, Pakistan. Author
  • Dr. Umbreen Farrukh Assistant Professor, Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Dow College of Pharmacy, Dow University of Health Sciences, Karachi, Pakistan. Author
  • Nayab Zehra Department of Botany, Government College Women University Faisalabad, Pakistan. Author
  • Mahrukh Department of Botany, Government College Women University Faisalabad, Pakistan. Author
  • Sajid Ahmad Department of Pharmacy, Government College University Faisalabad Pakistan. Author
  • Muhammad Faizan Tariq M Phil Scholar, Department of Pharmacy Government College University Faisalabad Pakistan Author
  • Dr. Rabbia Iqbal Department of Pharmacy, The University of Faisalabad (TUF), Faisalabad Pakistan Author

Keywords:

Lactuca sativa, gas chromatography-mass spectrometry (GC-MS), antioxidant activity.

Abstract

 The isolation and chemical analysis of Lactuca sativa L (L. sativa) seeds oil and its free radical scavenging activity was carried out. Soxhlet extraction method was used to extract the oil. The free radical scavenging activity (DPPH) method was used to identify the antioxidant activity of the extract. Almost 33 different chemical compounds were identified from spectra by GC-MS including α-isoamylene, (z) 2-Pentene, (E) 2- pentene, f-isoamylene, Isoamyl methyl ketone, Methyl 2-methyl butyl ketone, 1-methyl-1- ethylcyclopentene, n-amyl methyl ketone, 5-Octandione, Isopropyl pentyl ketone, 2-amyl furan, Pentanoic acid, Hexanoic acid, 1-ethyl-1-methyl-cyclopentane, (E)-2-Octenal, Some Hexanoic acid ester, Octyl ester acid, Undecane, Trans-3-Nonen-2-one, Dodecane, Octyl alcohol ester, ethyl hexyl ester, Tri-decanol, C11H20O2, 2,4, Decadienal, C10H18O, 3-Decanone, 4-undecanone, 1-Tetradecanol, Tetradecane, Hexadecane, Octadecane, Nonadecane, Palmitic Acid. Chemical components of the essential oils from locally grown lettuce have been identified in the following class or group of chemical compounds: monoterpene, sesquiterpene volatile organic compounds, flavonol glycosides, and their oxygenated hydrocarbon compounds. Thus, essential oils and raw leaves are effective candidates for use as antifungal or antimicrobial agents in the formulation of new drugs for the treatment of infectious diseases. 

Downloads

Download data is not yet available.

References

Alonso-Castro, A.J., R. Serrano-Vega, S. Pérez Gutiérrez, M.A. Isiordia-Espinoza and C.R. SolorioAlvarado. 2022. Myristic acid reduces skin inflammation and nociception. J. Food Biochem.

:1–9.

Alqahtani, F.Y., F.S. Aleanizy, A.Z. Mahmoud, N.N. Farshori, R. Alfaraj, E.S. Al-sheddi and I.A.

Alsarra. 2019. Chemical composition and antimicrobial, antioxidant, and anti-inflammatory

activities of Lepidium sativum seed oil. Saudi J. Biol. Sci. 26:1089–1092.

Altunkaya, A., E.M. Becker, V. Gökmen and L.H. Skibsted. 2009. Antioxidant activity of lettuce

extract (Lactuca sativa) and synergism with added phenolic antioxidants. Food Chem. 115:163–

Amorati, R., M.C. Foti and L. Valgimigli. 2013. Antioxidant activity of essential oils. J. Agric. Food

Chem. 61:10835–10847.

Anilakumar, K.R., A. Pal, F. Khanum and A.S. Bawa. 2010. Nutritional, medicinal and industrial uses

of sesame (sesamum indicum L.) seeds - An overview. Agric. Conspec. Sci. 75:159–168.

Borovkov, V.I. 2008. Excess electrons scavenging in n-dodecane solution: The role of tunneling of

electron from its localized state to acceptor. Radiat. Phys. Chem. 77:1190–1197.

Caboni, P., N.G. Ntalli, N. Aissani, I. Cavoski and A. Angioni. 2012. Nematicidal activity of (E, E)-2,4-decadienal and (E)-2-decenal from Ailanthus altissima against Meloidogyne javanica. J.

Agric. Food Chem. 60:1146–1151.

Carta, G., E. Murru, S. Banni and C. Manca. 2017. Palmitic acid: Physiological role, metabolism and

nutritional implications. Front. Physiol. 8.

Chen, X., X. Zhao, Y. Deng, X. Bu, H. Ye and N. Guo. 2019. Antimicrobial potential of myristic acid

against Listeria monocytogenes in milk. J. Antibiot. (Tokyo). 72:298–305.

Chhikara, N., K. Kushwaha, P. Sharma, Y. Gat and A. Panghal. 2019. Bioactive compounds of beetroot

and utilization in food processing industry: A critical review. Food Chem. 272:192–200.

Cisneros-Pineda, O., L.W. Torres-Tapia, L.C. Gutiérrez-Pacheco, F. Contreras-Martín, T. GonzálezEstrada and S.R. Peraza-Sánchez. 2007. Capsaicinoids quantification in chili peppers cultivated in

the state of Yucatan, Mexico. Food Chem. 104:1755–1760.

Clandinin, M.T., S.L. Cook, S.D. Konard and M.A. French. 2000. The effect of palmitic acid on

lipoprotein cholesterol levels. Int. J. Food Sci. Nutr. 51:s61–s71.

F. Xu, G.A.Zou, Y.Q. Liu, H.A.A. 2012. Chemical Consituents From Seeds of Lactuca sativa. Chem.

Nat. Compd. 48:574–576.

Fauziah, R. R., Rie, C., Yoshino, T., Ogita, S., & Yamamoto, Y.Fauziah, R. R., Rie, C., Yoshino, T.,

Ogita, S., & Yamamoto, Y. 2022. Anti-Cancer Effect of Phosphatidylcholine Containing

Conjugated Linoleic Acid at sn-2 Position on MCF-7 Breast Cancer Cell Line. Indones. J. Sci.

Technol. 7:279–290.

Gad, H.A., F.A.A. Abd El-Rahman and G.M. Hamdy. 2019. Chamomile oil loaded solid lipid

nanoparticles: A naturally formulated remedy to enhance the wound healing. J. Drug Deliv. Sci.

Technol. 50:329–338.

Hefnawy, H.T.M. and M.F. Ramadan. 2013. Protective effects of Lactuca sativa ethanolic extract on

carbon tetrachloride induced oxidative damage in rats. Asian Pacific J. Trop. Dis. 3:277–285.

Horrocks, L.A. and Y.K. Yeo. 1999. Health benefits of docosahexaenoic acid (DHA). Pharmacol. Res.

:211–225.

Idris, O.A., O.A. Wintola and A.J. Afolayan. 2019. Comparison of the proximate composition,

Vitamins (Ascorbic acid, α-Tocopherol and retinol), anti-nutrients (phytate and oxalate) and the

GC-MS analysis of the essential oil of the root and leaf of Rumex Crispus l. Plants. 8:51.

Kapoor, B., D. Kapoor, S. Gautam, R. Singh and S. Bhardwaj. 2021. Dietary Polyunsaturated Fatty

Acids (PUFAs): Uses and Potential Health Benefits. Curr. Nutr. Rep. 10:232–242.

Kazaz, S., Baydar, H., & Erbas, S. 2009. Variations in Chemical Compositions. Czech J. Food Sci.

:178–184.

Kien, C.L., J.Y. Bunn and F. Ugrasbul. 2005. Increasing dietary palmitic acid decreases fat oxidation

and daily energy expenditure. Am. J. Clin. Nutr. 82:320–326.

Kim, M.J., H.J. Doh, M.K. Choi, S.J. Chung, C.K. Shim, D.D. Kim, J.S. Kim, C.S. Yong and H.G.

Choi. 2008. Skin permeation enhancement of diclofenac by fatty acids. Drug Deliv. 15:373–379.

Kumar, M.H., K. Prabhu, M.R.K. Rao, R.L. Sundram, S. Shil, M.S. Kumar and N. Vijayalakshmi.

The gas chromatography–mass spectrometry study of one medicinal plant, Aristolochia

indica. Drug Invent. Today. 12:2919–2923.

Lee, W. Y., Lee, C. Y., Kim, Y. S., & Kim, C.E. 2019. The methodological trends of traditional herbal

medicine employing network pharmacology. Biomolecules. 9:362.

de Lima, R. M. T., Dos Reis, A. C., de Oliveira Santos, J. V., de Oliveira Ferreira, J. R., de Oliveira

Filho, J. W. G., Dias, A. C. S., ... & de Carvalho Melo-Cavalcante, A.A. 2020. Antitumoral effects

of [6]-gingerol [(S)-5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-3-decanone] in sarcoma 180 cells

through cytogenetic mechanisms. Biomed. Pharmacother. 126:110004.

MacDonald, I., Omonhinmin, A. C., & Ogboghodo, I.A. 2002. Association of Official Seed Analysts

Society of Commercial Seed Technologists. Seed Technol. 24:103–107.

Nakamura, S., Y. Kimura, D. Mori, T. Imada, Y. Izuta, M. Shibuya, H. Sakaguchi, E. Oonishi, N.

Okada, K. Matsumoto and K. Tsubota. 2017. Restoration of tear secretion in a murine dry eye

model by oral administration of palmitoleic acid. Nutrients. 9:1–11.

Nomaani, R.S.S. Al, M.A. Hossain, A.M. Weli, Q. Al-Riyami and J.N. Al-Sabahi. 2013. Chemical

composition of essential oils and in vitro antioxidant activity of fresh and dry leaves crude extracts

of medicinal plant of Lactuca Sativa L. native to Sultanate of Oman. Asian Pac. J. Trop. Biomed.

:353–357.

Noumedem, J.A.K., D.E. Djeussi, L. Hritcu, M. Mihasan and V. Kuete. 2017. Chapter 20 - Lactuca

sativa. In: In Medicinal Spices and Vegetables from Africa, Academic Press, 437–449.

Pepe, G., F. Pagano, S. Adesso, E. Sommella, C. Ostacolo, M. Manfra, M. Chieppa, M. Sala, M. Russo,

S. Marzocco and P. Campiglia. 2017. Bioavailable Citrus sinensis extract: Polyphenolic

composition and biological activity. Molecules. 22:1–15.

Rashighi, M. and J.E. Harris. 2017. 乳鼠心肌提取HHS Public Access. Physiol. Behav. 176:139–148.

Ruzicka, T., T. Simmet, B.A. Peskar and J. Ring. 1986. Skin levels of arachidonic acid-derived

inflammatory mediators and histamine in atopic dermatitis and psoriasis. J. Invest. Dermatol.

:105–108.

Said-, H.A.H., A. Ahl, W. Hikal, K. Tkachenko, H.A.H. Said-Al, H.A.H. Said-Al Ahl, W.M. Hikal and

K.G. Tkachenko. 2017. Essential Oils with Potential as Insecticidal Agents: A Review. Int. J.

Environ. Plan. Manag. 3:23–33.

Sales-Campos, H., P. Reis de Souza, B. Crema Peghini, J. Santana da Silva and C. Ribeiro Cardoso. 2013. An Overview of the Modulatory Effects of Oleic Acid in Health and Disease. Mini-Reviews

Med. Chem. 13:201–210.

Sayyah, M., N. Hadidi and M. Kamalinejad. 2004. Analgesic and anti-inflammatory activity of Lactuca

sativa seed extract in rats. J. Ethnopharmacol. 92:325–329.

Sharaf, M. 2008. Chemical constituents from the seeds of Trifolium alexandrinum. Nat. Prod. Res.

:1620–1623.

Smeriglio, A., D. Trombetta, L. Cornara, M. Valussi, V. De Feo and L. Caputo. 2019. Characterization

and phytotoxicity assessment of essential oils from plant byproducts. Molecules. 24:1–16.

Smoleń, S., I. Kowalska, P. Kováčik, M. Halka and W. Sady. 2019. Biofortification of six varieties of

lettuce (Lactuca sativa l.) with iodine and selenium in combination with the application of

salicylic acid. Front. Plant Sci. 10:1–13.

Sokmen, B.B., H.C. Onar, A. Yusufoglu and R. Yanardag. 2012. Anti-elastase, anti-urease and

antioxidant activities of (3-13)-monohydroxyeicosanoic acid isomers. J. Serbian Chem. Soc.

:1353–1361.

Song, W., C.M. Derito, M.K. Liu, X. He, M. Dong and R.H. Liu. 2010. Cellular antioxidant activity of

common vegetables. J. Agric. Food Chem. 58:6621–6629.

Sularz, O., S. Smoleri, A. Koronowicz, I. Kowalska and T. Leszczynska. 2020. Chemical Composition

of Lettuce ( Lactuca sativa L .). Agronomy. 10:1–17.

Taga, M.S., E.E. Miller and D.E. Pratt. 1984. Chia seeds as a source of natural lipid antioxidants. J.

Am. Oil Chem. Soc. 61:928–931.

Teixeira, B., A. Marques, C. Ramos, C. Serrano, O. Matos, N.R. Neng, J.M.F. Nogueira, J.A. Saraiva

and M.L. Nunes. 2013. Chemical composition and bioactivity of different oregano (Origanum

vulgare) extracts and essential oil. J. Sci. Food Agric. 93:2707–2714.

Ullah, A., S. Munir, S.L. Badshah, N. Khan, L. Ghani, B.G. Poulson, A. Emwas and M. Jaremko. 2020.

Therapeutic Agent. Molecules. 25:5243.

Whigham, L.D., M.E. Cook and R.L. Atkinson. 2000. Conjugated linoleic acid: Implications for human

health. Pharmacol. Res. 42:503–510.

Woo, J.O., M. Misran, P.F. Lee and L.P. Tan. 2014. Development of a controlled release of salicylic

acid loaded stearic acid-oleic acid nanoparticles in cream for topical delivery. Sci. World J. 2014.

Xu, B. and S.K.C. Chang. 2008. Effect of soaking, boiling, and steaming on total phenolic contentand

antioxidant activities of cool season food legumes. Food Chem. 110:1–13.

Yakoot, M., Helmy and Fawal. 2011. Pilot study of the efficacy and safety of lettuce seed oil in patients

with sleep disorders. Int. J. Gen. Med.:451.

Downloads

Published

2024-02-29

How to Cite

Ali, I., Iqbal, S., BILALOV, M., Qazaf, M., Muddassar Maqsood, M., Farrukh, U., Zehra, N., Mahrukh, Ahmad, S., Faizan Tariq, M., & Iqbal, R. (2024). Phytochemical screening of oil of Lactuca sativa seeds and determination of important bioactive and physicochemical aspects. History of Medicine, 10(1). https://historymedjournal.com/HOM/index.php/medicine/article/view/713