INVESTIGATION OF THE EFFECT OF BORIC ACID IN DIFFERENT CONCENTRATIONS ON DENTIN MICROHARDNESS
Keywords:
Boric acid; endodontics; irrigation solution; microhardness.Abstract
Purpose: This study compared the effects of different irrigation solutions on the microhardness of root dentin. Methods: Fifty single-rooted human teeth were chosen, and the roots were randomly divided into five groups (n = 10) according to the irrigant used for 5 min: 17% ethylenediaminetetraacetic acid (EDTA), 2% boric acid (BA), 5% BA, 10% BA, and distilled water (DW). After the irrigation procedure, dentin surface microhardness was calculated using a Vickers indenter 100 μm from the root canal lumen. Comparisons between the groups were performed with a two-way ANOVA test and and Tukey’s multiple comparison test (p = 0.05). Results: All irrigation solutions decreased the microhardness of root canal dentin. The DW and 2% BA had a minimum effect on the microhardness of root canal dentin, whereas a significant decrease in surface microhardness was found in 10% BA group (p < 0.05). The coronal third of the 10% BA group showed the lowest percentage decrease, with a significant difference between the apical and middle thirds (p < 0.05). Conclusion: In this study, 5% BA and 17% EDTA showed similar effects on the microhardness of root canal dentin. Further clinical research is required to evaluate the biocompatibility and safety of BA solutions.
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References
Siqueira JF Jr. Aetiology of root canal treatment failure: why well-treated teeth can fail. Int
Endod J 2001; 34: 1–10. [CrossRef]
Hargreaves KM, Cohen S, Berman LH. Cleaning and shaping the root canal system. In:
Peters OA, Peters CI, Basrani B, editors. Cohen’s Pathways of the Pulp. St. Louis, Mo: Mosby
Elsevier; 2012. p. 209–13.
Çiçek E, Özerol NB. Evaluation of two different root canal sealer, using different irrigation
solution on push out bond strenght to dentin. [Article in Turkish]. J Dent Fac Atatürk Uni
; 24: 16–21.
Sen BH, Wesselink PR, Turkun M. The smear layer: a phenomenon in root canal therapy.
Int Endod J 1995; 28: 141–8. [CrossRef]
Chandler N, Chellappa D. Lubrication during root canal treatment. Aust Endod J 2019; 45:
–10. [CrossRef]
Ostby N. Chelating in root canal therapy. Ethylene-di amine tetraacetic acid for cleansing
and widening of root canals. Odontol Tidskr 1957; 65: 3–11.
Violich DR, Chandler NP. The smear layer in endodontics - a review. Int Endod J 2010; 43:
–15. [CrossRef]
Calt S, Serper A. Time-dependent effects of EDTA on dentin structures. J Endod 2002; 28:
–9. [CrossRef]
Cobankara FK, Erdogan H, Hamurcu M. Effects of che lating agents on the mineral content
of root canal dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011; 112: 149–54.
[CrossRef]
Turk T, Kaval ME, Şen BH. Evaluation of the smear layer removal and erosive capacity of
EDTA, boric acid, citric acid and desy clean solutions: an in vitro study. BMC Oral Health 2015;
: 104. [CrossRef]
Kanoriya D, Singhal S, Garg V, et al. Clinical efficacy of subgingivally-delivered 0.75% boric
acid gel as an adjunct to mechanotherapy in chronic periodontitis: a randomized, controlled
clinical trial. J Investig Clin Dent 2018; 9.
Quarles W. Boric Acid, Borates and Household Pests. The IPM Practioner. Bio Integral
Research Center, Berkeley, CA. 2001; 23: 1–12.
Ince S, Kucukkurt I, Cigerci IH, et al. The effects of dietary boric acid and borax
supplementation on lipid peroxida tion, antioxidant activity, and DNA damage in rats. J Trace
Elem Med Biol 2010; 24: 161–4. [CrossRef]
Akman M, Belli S, Olcay K, et al. The effect of boric acid on root dentin mineral content
and bond strength of AH Plus: a SEM-EDX study. Turkiye Klinikleri J Dental Sci 2016; 22: 14–
[CrossRef]
Zan R, Hubbezoglu I, Ozdemır AK, et al. Antibacterial effect of different concentration of
boric acid against en terococcus faecalis biofilms in root canal. Marmara Dent J 2013; 2: 76–
Culhaoglu AK, Özcan E, Kilicarslan MA, et al. Effect of boric acid versus conventional
irrigation solutions on the bond strength between fiber post and root dentin. J Adhes Dent
; 19: 137–46.
Ari H, Erdemir A, Belli S. Evaluation of the effect of end odontic irrigation solutions on
the microhardness and the roughness of root canal dentin. J Endod 2004; 30: 792–5.
Cruz-Filho AM, Sousa-Neto MD, Savioli RN, et al. Effect of chelating solutions on the
microhardness of root canal lumen dentin. J Endod 2011; 37: 358–62. [CrossRef]
Bosaid F, Aksel H, Makowka S, et al. Surface and struc tural changes in root dentine by
various chelating solutions used in regenerative endodontics. Int Endod J 2020; 53: 1438–45.
[CrossRef]
Akbulut MB, Guneser MB, Eldeniz AU. Effects of fruit vinegars on root dentin
microhardness and roughness. J Conserv Dent 2019; 22: 97–101.
Baldasso FER, Roleto L, Silva VDD, et al. Effect of final irrigation protocols on
microhardness reduction and erosion of root canal dentin. Braz Oral Res 2017; 31: e40.
[CrossRef]
Arun DR, Sujatha V, Mahalaxmi S. Effect of 5% carbo hydrate derived-fulvic acid on smear
layer removal and root dentin microhardness - an in vitro study. Eur Endod J 2022; 7: 156–60.
Muana HL, Nassar M, Dargham A, et al. Effect of smear layer removal agents on the
microhardness and roughness of radicular dentin. Saudi Dent J 2021; 33: 661–5. [CrossRef]
Hennequin M, Pajot J, Avignant D. Effects of different pH values of citric acid solutions on
the calcium and phos phorus contents of human root dentin. J Endod 1994; 20: 551–4.
[CrossRef]
Dogan H, Qalt S. Effects of chelating agents and sodium hypochlorite on mineral content
of root dentin. J Endod 2001; 27: 578–80. [CrossRef]
Das A, Kottoor J, Mathew J, et al. Dentine microhard ness changes following conventional
and alternate irriga tion regimens: an in vitro study. J Conserv Dent 2014; 17: 546–9.
[CrossRef]
Sayin TC, Serper A, Cehreli ZC, et al. Calcium loss from root canal dentin following EDTA,
EGTA, EDTAC, and tetracycline-HCl treatment with or without subsequent NaOCl irrigation. J
Endod 2007; 33: 581–4. [CrossRef]
Gutiérrez-Salazar MdP, Reyes-Gasga J. Microhardness and chemical composition of
human tooth. Mater Res 2003; 6: 367–73. [CrossRef]
Lottanti S, Gautschi H, Sener B, et al. Effects of ethylene diaminetetraacetic, etidronic
and peracetic acid irrigation on human root dentine and the smear layer. Int Endod J 2009;
: 335–43. [CrossRef]
Goldberg F, Spielberg C. The effect of EDTAC and the variation of its working time
analyzed with scanning elec tron microscopy. Oral Surg Oral Med Oral Pathol 1982; 53: 74–7.
[CrossRef]
De-Deus G, Paciornik S, Mauricio MH. Evaluation of the effect of EDTA, EDTAC and citric
acid on the microhard ness of root dentine. Int Endod J 2006; 39: 401–7. [CrossRef]
Ulusoy OI, Gorgul G. Effects of different irrigation solu tions on root dentine
microhardness, smear layer removal and erosion. Aust Endod J 2013; 39: 66–72. [CrossRef]
Saleh AA, Ettman WM. Effect of endodontic irrigation solutions on microhardness of root
canal dentine. J Dent 1999; 27: 43–6. [CrossRef]
Saha SG, Sharma V, Bharadwaj A, et al. Effectiveness of various endodontic irrigants on
the micro-hardness of the root canal dentin: an in vitro study. J Clin Diagn Res 2017; 11:
ZC01–4. [CrossRef] 36. Aranda-Garcia AJ, Kuga MC, Chavéz-Andrade GM, et al. Effect of final
irrigation protocols on microhardness and erosion of root canal dentin. Microsc Res Tech
; 76: 1079–83. [CrossRef]
Tsenova-Ilieva I, Karova E. Effect of endodontic irrigants on root dentin microhardness: a
systematic review. Int J Sci Res 2020; 9: 491–6.
Belizario LG, Piragine TL, Girotto AC, et al. Effect of dif ferent irrigants on the adhesive
interface and influence on the push out strength of fiber posts. Oper Dent 2022; 47: 211–21.
[CrossRef]
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