Molecular Detection for Some Virulence Factors of Salmonella typhi from Iraqi Patients

Authors

  • Menan Abdul-Razaq Abdul-Khadum College of Education for Pure Science- Diyala University, Iraq Author
  • Ali Jaffar Saleem College of Education for Pure Science- Diyala University, Iraq. Author
  • Amal Aziz Karim Middle Technical University, College of Health and medical Technology, Research unite, Iraq Author

Keywords:

Salmoella typhi ,16SrRNA, cdtB, tviA, fimA and orfL genes, Virulence Factors genes.

Abstract

In current study, 29 clinical isolates Salmonella typhi were collected from different Private laboratories in Diyala. In addtion of phenotypic method, all isolates diagnosed as S. typhi by genotypic method was done by Polymerase Chain Reaction (PCR) technique to detection 16SrRNAgene. And detection of adhesion factors associated genes that encoding for cdtB, tviA, fimA and orfL by using specific primers which showed the prevalence in percentage 100% for all genes. 

Downloads

Download data is not yet available.

References

MacFaddin, J.F. (2000). Biochemical Tests for

Identification of Medical Bacteria. 3rd ed. Lippincott

Williams and Wilkins, USA.

ALJobouri, Abdul Aziz T.A.F(2019).Molecular

Characterization of Some Virulence Genes of

Salmonella enterica in Babylon province ,Thesis M.S.C,

Medicine, University of Babylon.

Abbas, Hussein Hafidh (2012). Detection of Salmonella

enterica serovar typhi in patients with acute and chronic

typhoid by some bacteriological, immunological and

PCR technique , Thesis M.S.C, College of Science,

University of Baghdad.

Johnson, R. (2018). Investigating the globally dominant

Salmonella Typhi haplotype H58–an insight into the

pathogenesis of typhoid fever.

AL-Oqaili, NAD (2019), Detection of some virulence factors

of Salmonella typhi isolated from patients’ blood by

PCR and phylogenetic tree, Periodicals of Engineering

and Natural Sciences, 7(4) pp. 1915–1923. doi:

21533/pen.v7i4.941.

LEGBA, B.,Dougnon, T. V., DEGUENON, E., HOUNMANOU,

G., AGBANKPE, J., AMADOU, A., ... & DOUGNON, T. J.

(2017). Pathogenicity, epidemiology and virulence

factors of Salmonella species: A review. Notulae

Scientia Biologicae, 9(4), 460-466.

Cohen, H. J., Mechanda, S. M., & Lin, W. (1996). PCR

amplification of the fimA gene sequence of Salmonella

typhimurium, a specific method for detection of

Salmonella spp. Applied and environmental

microbiology, 62(12), 4303-4308.

Khazaal, Saba Saadoon(2019). Detection Flagellin Gene of

Enteric Isolates of Salmonella enterica serovar Typhi

Using Conventional PCR Technique, Al-Mustansiriyah

Journal of Science,30(3): ISSN: 1814-635X (print),

ISSN:2521-3520 (online).

Albanwawy, Jihad N. Abid; Abdul-Lateef, , Lamees

A.(2021).Molecular Detection of Some of the Salmonella

Typhi Virulence Genes Isolated in the Province of

Babylon/Iraq. Annals of R.S.C.B., ISSN: 1583-6258 ,25(2).

– 685.

Baker, S. ; Sarwar, Y.; Aziz, H.; Haque, A.; Ali, A.; Dougan, G.;

Wain, J. and Haque, A. (2005). Detection of Vi-negative

Salmonella enterica serovar typhi in the peripheral

blood of patients with typhoid fever in the Faisalabad

region of Pakistan. J Clin Microbiol .43(9):4418-25. doi:

1128/JCM.43.9.4418-4425.2005. PMID: 16145086;

PMCID: PMC1234127.

Liaquat, S. et al. (2018) ‘Virulotyping of Salmonella enterica

serovar Typhi isolates from Pakistan: Absence of

complete SPI-10 in Vi negative isolates’, PLoS Neglected

Tropical Diseases, 12(11), pp. 1–20. doi:

1371/journal.pntd.0006839.

AL-Khafaji, Angham Najah (2022). Occurrences and

Virulence Genes Detection of Salmonella Typhi Isolates

Among Patients with Typhoid Fever. Al-Furat Al-Awsat

Technical University. https://orcid.org/0000-0002-

-1905.

Gassama-Sow, A. et al. (2006) ‘Characterization of

virulence factors in the newly described Salmonella

enterica serotype Keurmassar emerging in Senegal

(sub-Saharan Africa)’, Epidemiology and Infection,

(4), pp. 741–743. doi: 10.1017/S0950268805005807.

Ramatla,T. A.; Mphuthi, N.; Ramaili, T.; Taioe,M.; Thekisoe,O.

,and Syakalima, M.(2022). Molecular detection of

zoonotic pathogens causing gastroenteritis in humans

Salmonella spp., Shigella spp. and Escherichia coli

isolated from Rattus species inhabiting chicken farms in

North West Province, South Africa. J S Afr Vet Assoc.

Online ahead of print

https://doi.org/10.36303/JSAVA.83.

Santos, R. I., Tsolis, R. M.,Baumler, A. J.,and Adams,L.

G.(2003).Pathogenesis of Salmonella – induced

enteritis. Brazilian Journal of Medical and Biological

Research.36(1),03-12.

Santander M.J., Roland K.L., Curtiss III R., (2008)

.Regulation of Vi Capsular Polysaccharide Synthesis in

Salmonella enteric Serotype Typhi J. Infect. Developing

Countries, 2, 412-420.

Song, J., Gao, X. and Galán, J. E. (2013): Structure and

function of the Salmonella Typhi chimaeric A(2)B(5)

typhoid toxin. Nature 499, 350–4.

Kolenda ,R; Ugorski, M and Grzymajlo, K(2019). Everything

You Always Wanted to Know About Salmonella Type 1

Fimbriae, but Were Afraid to Ask, Front. Microbiol.

:1017. Doi: 10.3389/fmicb.2019.01017.

World Health Organization (WHO). Typhoid vaccines

(2018).Typhoid and other invasive salmonellosis

;(http://apps.who.in/iris/bitstream/handle/10665/2722

/WER9313.pdf?ua=1).

Wong ,W. ;Al Rawahi ,H. ;Patel ,S. ;Yau, Y.;Eshaghi,

A.;Zittermann, S.; Tattum, L. and Morris, Sh

.K.(2019).The first Canadian pediatric case of

extensively drug- resistant Salmonella Typhi originating

from an outbreak in Pakistan and it’s the implication for

empiric antimicrobial choices, Published by Elsevier

I.td;https://doi.org/10.10 16/ j.idcr.2019 .e00492.

NCBI-a :

https://www.ncbi.nlm.nih.gov/protein/CHO04914.1

NCBI-b: https://www.ncbi.nlm.nih.gov/protein/UBI83717.1

NCBI-c: https://www.ncbi.nlm.nih.gov/protein/QKX99054.1

Suardana, I. W. (2014). Analysis of Nucleotide Sequences of

the 16SrRNA Gene of Novel Escherichia coli Strains

Isolated from Feces of Human and Bali Cattle. J.

Nucleic Acids : 1-7.

Srinivasan, R.; Karaoz, U.; Volegova, M.; MacKichan, J.;

KatoMaeda, M.; Miller, S.; Nadarajan , R.; Brodie , E. L.

and Lynch, S. V. (2015). Use of 16SrRNA Gene for

Identification of a Broad Range of Clinically Relevant

Bacterial Pathogens . PLOS ONE. 10(2): 1-22.

Grimont, P. A. D.; Grimont, F.; and Bouvet, P. (2000).

Taxonomy of the Genus Salmonella. In: Wray, C., and

Wray A. (Eds.). Salmonella in Domestic Animals. CABI

Publishing, Wallingford, UK. Pp.:1-17.

AL-Muhannak ,Fadhil Hussain Nasir( 2020),Antibiotic

Susceptibility and Molecular Characterization of

Salmoneiia Typhi Isolated from Blood Samples in Nagaf ,Thesis ,Medical Microbiology, University of Kufa / Facuity of Medicine,15 :163.

Zhang , Yin et al.(2018).Reciprocal Regulation of OmpR and Hfq and Their Regulatory Actions on the Vi polysaccharide Capsular Antigen in Salmonella enterica Serovar Typhi . Current Microbiology, 75(6), pp.773- 778.doi: 10.1007/s00284-018-1447-7.

Downloads

Published

2023-04-30

Issue

Section

Articles

How to Cite

Abdul-Razaq Abdul-Khadum, M., Jaffar Saleem, A., & Aziz Karim, A. (2023). Molecular Detection for Some Virulence Factors of Salmonella typhi from Iraqi Patients. History of Medicine, 9(2). https://historymedjournal.com/HOM/index.php/medicine/article/view/609