A comparative study of Integrin-linked kinase 1 and anti-smith antibody as diagnostic biomarkers in Iraqi patients with systemic lupus erythematosus

Authors

  • Zaman Badary Department of Chemistry, Collage of Education for Pure Sciences ibn Al-Haitham University of Baghdad, Baghdad, Iraq Author
  • Eiman A. A. Abass Department of Chemistry, Collage of Education for Pure Sciences ibn Al-Haitham University of Baghdad, Baghdad, Iraq Author

Keywords:

systemic lupus erythematosus, integrin-linked kinase 1, anti-smith antibody, lupus nephritis

Abstract

Systemic lupus erythematosus (SLE) is the prototypical multisystem autoimmune disease, with a broad spectrum of clinical manifestations affecting virtually all organs and tissues. Determination of integrin-linked kinase 1 (ILK- 1) and anti-smith antibody (ASAB) levels in serum of Iraqi patients with systemic lupus erythematosus; that ILK-1 may serve as a diagnostic marker for SLE disease; and evaluation of the impact of systemic lupus erythematosus on renal function in these patients. This study included 100 female patients with systemic lupus erythematosus from the Rheumatology Unit at the Baghdad Teaching Hospital, Medical City, (Baghdad), as well as 30 healthy females without any chronic diseases who served as the control group. All study participants’ ages ranged between 18 and 48 years. Using Rheuma Helper, all patients were classified based on the SLE disease activity index (SLEDAI) severity into 30 female patients with mild stage (Gl), 40 female patients with moderate stage (G2), and 30 female patients with severe stage (G3). (G3). Results: As compared to the control (C) group, the Gl, G2, and G3 patient groups in the current investigation showed a substantial increase in the levels of dsDNA, ANA, ASAB, ILK-1, urea, and uric acid. Comparing the control group to Gl, G2, and G3, the levels of creatinine did not differ. The current results showed that there were various significant comparisons between ILK-1 and other study parameters in the control and patient groups. ROC test for ILK-1 and ASAB markers showed a perfect cut-off value with 100% sensitivity and 100% specificity. Conclusions: TK-1 may be helpful as a novel biomarker in the diagnosis of SLE disease, and there was a positive correlation between ILK-1 with urea and creatinine which are considered factors of renal damage. As a result, these patients may be at risk for renal failure at a severe stage. This indicates that SLE disease has an impact on kidney function. 

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References

I.Zaidan, T.F., Oral manifestations, oral health status and

saliva composition changes in a sample of Iraqi

systemic lupus erythematosus patients. Scientific

Journal Published by the College of DentistryUniversity of Baghdad, 2012: p. 65.

Tawfeq, A., A.F. Al-Rubaye, and M.M.H. AL-Murshidi,

Cross Sectional Study of the Correlation between ESR,

RBCS and WBCs with Disease Duration in Iraqi

Patients with Systemic Lupus Erythematosus Disease

(SLE). Indian Journal of Forensic Medicine & Toxicology,

14(3): p. 2579-2583.

Abdulridha, R.H., A.M. Saud, and M.H. Alosami, Evaluation

of Interferon Alpha (IFN- ) in Women with Systemic

Lupus Erythematosus in Iraq. Iraqi Journal of Science,

: p. 4225-4233.

Rees, F., et al., The worldwide incidence and prevalence of

systemic lupus erythematosus: a systematic review of

epidemiological studies. Rheumatology, 2017. 56(11): p.

-1961.

Dahham, Z.M. and N.I. Haddad, Circulating Levels of

Interferon Regulatory Factor-5 Correlates with Disease

Activity in Systemic Lupus Erythematosus Iraqi

Patients. J Contemp Med Sci| Vol, 2022. 8(6): p. 388-

Migliorini, P., et al., Anti-Sm and anti-RNP antibodies.

Autoimmunity, 2005. 38(1): p. 47-54.

Zieve, G.W. and P.R. Khusial, The anti-Sm immune

response in autoimmunity and cell biology.

Autoimmunity reviews, 2003. 2(5): p. 235-240.

G6rska, A. and A.J. Mazur, Integrin-linked kinase (ILK):

the known vs. the unknown and perspectives. Cellular

and Molecular Life Sciences, 2022. 79(2): p.100.

Wickstrdm, S.A., et al., The ILK/PINCH/parvin complex:

the kinase is dead, long live the pseudokinase! The

EMBO journal, 2010. 29(2): p. 281-291.

lO.Kretzler, M., et al., Integrin linked kinase as a candidate

downstream effector in proteinuria. The FASEB

Journal, 2001.15(10): p. 1843-1845.

Ali, A. and S. Al-Windawi, Tubulointerstitial lupus

nephritis. Journal of nephropathology, 2013. 2(1): p. 75.

Parikh, S.V., et al., Update on lupus nephritis: core

curriculum 2020. American Journal of Kidney Diseases,

76(2): p. 265-281.

Anders, H.-J., et al., Lupus nephritis (Primer). Nature

Reviews: Disease Primers, 2020. 6(1).

Petri, M., E. Barr, and L.S. Magder, Risk of renal failure

within 10 or 20 years of systemic lupus erythematosus

diagnosis. The Journal of rheumatology, 2021. 48(2): p.

-227.

Bertsias, G., R. Cervera, and D.T. Boumpas, Systemic

lupus erythematosus: pathogenesis and clinical

features. EULAR textbook on rheumatic diseases, 2012.

: p. 476-505.

Gorial, F.I., Z.A. Mahmood, and S. Al Obaidi, Body

composition in Iraqi Women with systemic lupus

erythematosus. Glob J Health Sci, 2019. 11(1): p. 63-70.

Andrade, R.M., et al., Accelerated damage accrual

among men with systemic lupus erythematosus: XLIV.

Results from a multiethnic US cohort. Arthritis &

Rheumatism: Official Journal of the American College

of Rheumatology, 2007. 56(2): p. 622-630.

Barcia Sixto, L. and D. Isenberg, Systemic lupus

erythematosus: causes and manifestations. Trends in

Urology & Men's Health, 2020.11(1): p. 26-29.

Pashnina, I.A., et al., Antinuclear autoantibodies in

health: autoimmunity is not a synonym of autoimmune

disease. Antibodies, 2021.10(1): p. 9.

O.Abdualkader, S.N., et al., Correlation between

lnterleukin-4 and lnterleukin-6 and auto antibodies in

Systemic Lupus Erythematosus. Journal of the Faculty

of Medicine Baghdad, 2009. 51(4): p. 416-418.

Arbuckle, M.R., et al., Development of autoantibodies

before the clinical onset of systemic lupus

erythematosus. New England Journal of Medicine,

349(16): p. 1526-1533.

van Beers, J.J. and M.W. Schreurs, Anti-Sm antibodies in

the classification criteria of systemic lupus

erythematosus. Journal of Translational Autoimmunity,

: p. 100155.

Elustondo, P., et al., Integrin-linked kinase (ILK) is highly

expressed in first trimester human chorionic villi and

regulates migration of a human cytotrophoblastderived cell line. Biology of reproduction, 2006. 74(5): p.959-968.

Ton, C., A. Mui, and S. Dedhar, Integrin-linked kinase

regulates inducible nitric oxide synthase and

cyclooxygenase-2 expression in an NF- B-dependent

manner. Journal of Biological Chemistry, 2002. 277(5):

p. 3109-3116.

Ahmed, A.U., et al., Integrin-linked kinase expression in

myeloid cells promotes inflammatory signaling during

experimental colitis. The Journal of Immunology, 2017.

(6): p. 2128-2139.

de Frutos, S., et al., Chronic kidney disease induced by an

adenine rich diet upregulates integrin linked kinase (ILK)

and its depletion prevents the disease progression.

Biochimica et Biophysica Acta (BBA)-Molecular Basis of

Disease, 2019. 1865(6): p. 1284-1297.

Ibrahim, N., et al., Estimation of some immunological

markers of Iraqi patients in systemic lupus

erythematosus with lupus nephritis. Prof.(Dr) RK

Sharma, 2020. 20(4): p. 4668.

Cervera, R., Systemic lupus erythematosus in Europe at

the change of the millennium: lessons from the "Euro¬

Lupus Project". Autoimmunity reviews, 2006. 5(3): p.

-186.

AL-Qubaeissy, K.Y., et al., Does Rheumatoid Factor have

any Protective Role in Patients with Lupus Nephritis.

Editorial Board, 2020.

Block, S., A brief history of twins. 2006, Sage

Publications Sage CA: Thousand Oaks, CA. p. 61-64.

Yang, Z., et al., Associations of serum urea, creatinine and uric acid with clinical and laboratory features in patients with systemic lupus erythematosus. Rheumatology international, 2012. 32: p. 2715-2723.

Sham, S., A Study on QT Interval in patients with SLE and its Correlation with disease activity and Auto Antibodies. 2014, Madras Medical College, Chennai.

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Published

2023-04-30

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How to Cite

Badary, Z., & A. A. Abass, E. (2023). A comparative study of Integrin-linked kinase 1 and anti-smith antibody as diagnostic biomarkers in Iraqi patients with systemic lupus erythematosus. History of Medicine, 9(2). https://historymedjournal.com/HOM/index.php/medicine/article/view/592