Evaluation of the effect of biosynthetic zinc oxide by VITEX _ agnus castus L on the gene expression of the Quorum sensing genes
Keywords:
real-time, Vitex agnus L, gene expression, citramide, resazurinAbstract
The current study was conducted to investigate the effect of zinc oxide nanoparticles (ZnO—np) prepared by physical method, alcoholic extract of Vitex agnus-castus leaf and biosynthetic ZnO-np by using Vitex agnus-castus extracts on the gene expression of Quorum sensing (QS) system of biofilm-producing Pseudomonas aeruginosa. 100 samples were collected from clinical sources and after the identification, 18 clinical isolates were confirmed to be Pseudomonas aeruginosa by PCR using gyrB gene. The effect of MIC for all substances against four biofilmproducing isolates were examined by the microtiter plate method. Furthermore, qRT-PCR was performed to determine three substances inhibitory effect on QS-regulatory genes lasR, , rhlR, and pqsR that control virulence factors secretion. Biosynthetic ZnO—np, ZnO—np and plant extract showed significantly down-regulated the expression level of all biofilm and virulence genes of P. aeruginosa clinical isolates and thus we conclude that Biosynthetic ZnO-np can be used as a quorum sensing inhibitor and an anti-biofilm in P. aeruginosa infections.
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References
Bohodor , N .; Shojo , S .; Faridi , F Dozondeh - Moborrez ,
B .; Qeshmi , F .; Jovodpour , S .; and Mokhtary , S. (
) . Molecular detection of virulence factors and
biofilm formation In Pseudomonas aeruginosa obtained
from different . clinical specimens in bandar abbas .
Iranian Journal of Microbiology , 11 ( 1) : 25-30
Pachori P, Gothalwal R, Gandhi P. Emergence of antibiotic
resistance Pseudomonas aeruginosa in intensive care
unit; a critical review. Genes Dis. 2019 Apr 17;6(2):109-
doi: 10.1016/j.gendis.2019.04.001. PMID: 31194018;
PMCID: PMC6545445.
Pillai , A. M. , Sivasankarapillai , V. S. , Rahdar , A. , Joseph
, J., Sadeghfar , F. , Rajesh , K. , & Kyzas , G. Z. ( 2020 ) .
Green synthesis and characterization of zinc oxide
nanoparticles with antibacterial and antifungal activity
. Journal of Molecular Structure ,1211 , 128107 .
Fehan , H. J. , Mansoor , J. M. , & Habeeb , A. A. ( 2021 ) .
Production of Zirconium Oxide ( ZrO2 ) Micro and
Nanoparticles by Pulsed Laser Ablation in Liquid. Diyala
Journal For Pure Science , 17 ( 02 )
Othman , N. , Babakir - Mina , M. , Noori , C. K., and Rashid ,
P. Y. ( 2014 ) . Pseudomonas aeruginosa infection in
burn patients in Sulaimaniyah , Iraq : risk factors and
antibiotic resistance rates . Journal of Infection in
Developing Countries . 8 ( 11 ) :1498-1502.
AL - Sheikhly MARH , Musleh LN , Al - Mathkhury HJF (
) Gene expression of pelA and psIA in
Pseudomonas aeruginosa under gentamicin stress .
Iraqi J. Sci. 61(2), 295-305. 3
Shields , P. , and Cathcart , L. ( 2010 ) . Oxidase Test
Protocol . American Society for Microbiology.1-9.
.Tawfiq , S.M. , 2018. Bacteriologigal and genetic study of
Pseudomonas aeruginosa isolates . Iraqi Journal of
Agricultural Sciences , 49 ( 1) ,: 27-35 . 31. .
Abdul Amir Abdullah,2015. Study of the biological efficacy
of some extracts of chasteberry plant (vitex
agnus_castus L).Iraqi Journal of Science, 56(2),
:406_397
Sailman R S , Hussein S S and Ali M R ( 2018 ) ERIC- PCR
typing , RAPD - PCR fingerprinting and quorum sensing
gene analysis of Pseudomonas aeruginosa isolated
from different clinical sources . Al - Mustansiriyah J. Sci
. 29 ( 2 ) , 50.
AL - Gbouri , N.M. and A.M., Hamzah , 2018 . Evaluation of
phyllanthus emblica extract as antibacterial and
antibiofilm against biofilm formation bacteria . Iraqi
Journal of Agricultural Sciences , 49 ( 1) : 142-151
Dombek C,(1998). Ed. Lawerence Anonymous. Chaste Tree
Review of Natural Products. St. Louis: Facts and
Comparisons.
E. J. Lowbury and A. G. Collins , J. Clin. Pathol., 1955, 8 , 47
—48 CrossRef CAS PubMed .)
Jaffar , N. , Miyazaki , T. , and Maeda , T. ( 2016 ) . Biofilm
formation of periodontal pathogens on hydroxyapatite
surfaces : Implications for periodontium damage .
Journal of Biomedical Materials Research Part A. 104 (
) : 2873-2880.
Desjardins , P. , and Conklin , D. ( 2010 ) . NanoDrop
microvolume quantitation of nucleic acids . Journal of
Visualized Experiments. 45 :1-5
Tacconelli E. Carrara E , Savoldi A. Harbarth S , Mendelson
M , Monnet DL , et al . Discovery , research , and
development of new antibiotics : the WHO priority list
of antibiotic - resistant bacteria and tuberculosis .
Lancet Infect Dis . 2018 ;18 ( 3 ) : 318-327
Garcia - Lara B , Saucedo - Mora MA , Roldan - SanchezJA
, Perez - EretzaB , Ramasamy M , Lee J , et al .
Inhibition of quorum - sensing - dependent virulence
factorsand biofilm formation of clinical and
environmental Pseudomonas aeru ginosa strains by
ZnO nanoparticles . Letters in Applied Mi crobiology .
; 61 : 299-305.
Aysa NH , SalmanHD . Antibacterial activity of modi fied
zinc oxide nanoparticles against Ps . aeruginosa isolates
of burn infections . World Scientific News . 2016 ; 33 : 1-
Pati R , Mehta RK , Mohanty S , Padhi A , Sengupta M ,
Vaseeharan B , et al . Topical application of zinc oxide
nanoparticles reduces bacterial skin infection in mice
and exhibits antibacterial activity by inducing oxidative
stress response and cell membrane disintegration in
macro phages . Nanomedicine.2014 ;10 ( 6 ) :1195-208 .
Saleh MM , Sadeg RA , Alvdel Latif HK , Abbur HA , Askaana
M. Zinc wade nanoparticles inhibits quorum sencing and
verulence in Pseudomonas aeruginosa . Afri Health
Sa.2019 ;19 ( 2 ): 2043-2055
Jwan S. Bajlun , Harith J. F. Al - Mathkhury and Sahar G.
Imran ( 2022 ) Biofilm development capacity and
antibiotic susceptibility of Pseudomonas aeruginosa
from burn patients in Baghdad , Iraq . Biochem . Cell .
Arch . 22,1207-1211. DoelD.
.Teh CH, Nazni WA, Nurulhusna AH, Norazah A, Lee HL.
Determination of antibacterial activity and minimum inhibitory concentration of larval extract of fly via
resazurin-based turbidometric assay. BMC Microbiol.
Feb 16;17(1):36. Doi: 10.1186/s12866-017-0936-3.
PMID: 28209130; PMCID: PMC5314469.
Donlan RM. Biofilms: microbial life on surfaces. Emerg
Infect Dis. 2002 Sep;8(9):881-90. Doi:
3201/eid0809.020063. PMID: 12194761; PMCID:
PMC2732559.
M. A. Alaa Alden and L. A. Yaaqoob,2022. EVALUATION OF
THE BIOLOGICAL EFFECT SYNTHESIZED ZINC OXIDE
NANOPARTICLES ON PSEUDOMONAS AERUGINOSA
.Iraqi Journal of Agricultural Sciences,53 ( 1) : 27-37
.Abdallah Habbab , Khaled Sekkoum , Nasser Belboukhari ,
Abdelkrim Cheriti and Hassan Y. Aboul - Encin, 2017.
Analgesic and Anti - Inflammatory Effects of Essential
Oils of Vitex agnus castus L. from South - West of
Algeria.Bentham Science Publishers.
.SH.A.ALwendawi, and L.A.Charb, 2021. ANTIOXIDANT ,
ANTIBACTERIAL AND ANTIBIOFILM POTENTIALS OF
ANISE ( Pimpinella anisum ) SEEDS EXTRACTED
ESSENTIAL OILS.Iraqi Journal of Agricultural Sciences,
(2) : 338_358
Arabestani MR . Rajabpour M , Yousef Mahouf R. Alahani
MY Mousavi SM Expression of Efflus Pimp MexAB - Op
- M and OprD ) of P domonas aeruginosa Strains
Isolated from Clinical Samples uning qRT - PCR Arch
Iran Med . 2015 , 18 ( 2 ) : 102-108
.Whiteley M, Bangera MG, Bumgarner RE, Parsek MR,
Teitzel GM, Lory S, Greenberg EP. Gene expression in
Pseudomonas aeruginosa biofilms. Nature. 2001 Oct
;413(6858):860-4. Doi: 10.1038/35101627. PMID:
Kafil , H .; Mobarez , A .; Moghadam M .; Hashemi , Z .; and
Yousefi , M. ( 2016 ) Gentamicin induces efaA expression
and biofilm formation In Enterococcus faecalis .
Microbial Pathogenesis .92:30 35.
.Anuj SN, Whiley DM, Kidd TJ, Bell SC, Wainwright CE,
Nissen MD, Sloots TP. Identification of Pseudomonas
aeruginosa by a duplex real-time polymerase chain
reaction assay targeting the ecfX and the gyrB genes.
Diagn Microbiol Infect Dis. 2009 Feb;63(2):127-31. Doi:
1016/j.diagmicrobio.2008.09.018. Epub 2008 Nov 21.
PMID: 19026507.
Piewngam P, Chiou J, Chatterjee P, Otto M. Alternative
approaches to treat bacterial infections: targeting
quorum-sensing. Expert Rev Anti Infect Ther. 2020
Jun;18(6):499-510. Doi: 10.1080/14787210.2020.1750951.
Epub 2020 Apr 13. PMID: 32243194.
Shebab ZH , Ahmed ST , Abdallah NM ( 2020 ) : Genetic
variation of pill gene in Pseudomonas aeruginosa
isolated from Iraqi patients with bum infections , Ann
Trop Med & Public Health ; 23 ( S16 ) : SP231615 . DOI :
http://doi.org/10.36295/ASRO.2020.231615
.Usha Verme , Smita Kulshreshtha and Khatri , P.K. 2018.
MDR Pseudomonas aeruginosa in Nosocomial Infection
: Burden in ICU and Burm Units of a Tertiary Care
Hospital Int J.Curr.Microbiol . App . Sci 111 ) 1267 1274.
Doi
Ali SG , Ansari MA , Alzohairy MA ( 2020 ) Effect of
Biosynthesized ZnO Nanoparticles on Multi - Drug
Resistant Pseudomonas Aeruginosa . Antibiotic ( Basel )
:260
Pati R , Mehta RK , Mohanty S , Padhi A , Sengupta M ,
Vaseeharan B ( 2014 ) Topical application of zinc oxide
nanoparticles reduces bacterial skin infection in mice
and exhibits antibacterial activity by inducing oxidative
stress response and cell membrane disintegration in
macrophages . Nanomedicine ;10 :1195-1208.
Ekundayo O , Laakso I , Holopainen M , Hiltunen R ,
Oguntimein R. undayo Kauppinen V , 1990. The chemical
composition and antimicrobial activity of the leaf oil of
Vitex agnus - castus. J Essent Oil Res 2 : www . 115-119.
.6Wedad, M, Abdelraheem.,Ebtism,S.2O21.The effect of
Zinc Oxide nanoparticles on Pseudomonas aeruginosa
biofilm formation and virulence genes expression. The
journal of infections in developing countries, 15(6),
pp.826_832
.Khudiar MM , Jessim Al , Azeez AZ , Fakhry SS , Alwash SJ , Farhan Yl , Abdulbagi AA . ( 2016 ) Biofilm formation Staphylococcus spp . isolated from local food markets at Baghdad city . World J Exp Biosci 4: 87-92
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