Evolution of virulence factors and defection factor H.pylori among gastrointestinal disease patients in Waist province

Abstract

Helicobacter Pylori (H. pylori) infection is the most important factor affecting clinical outcome in patients with gastric mucosal lesions The aim of this study is to employ conventional PCR to determine the presence of Helicobacter pylori through molecular analysis of the 16S rRNA gene. Additionally, the existence of virulence genes, cagA, vacA, IceA, will be investigated.   Total sample was 40 positive h. Pylori infection in different stage range from mild until sever or chronic among gastrointestinal disease patient from waist province, the age of participant range from 12 to 62 years attended to gastrointestinal section in AL-karma testing hospital during 2022/2023. saliva spacemen was collected to Primers of Polymerase Chain Reaction (PCR) for detection of 16sRNA, CagA,and VagA sl/s2 as a detection and virulence factor of h.pylori infection.  The study found 16sRNA positive 40(100%), and CagA most of samples positive 23(57.5%), and the negative 17(42.5%), and the distribution of gastroenteritis patients according to VagA sl/s2 most of samples negative 32(80%) compare to positive 8(20%). there is no significant relationship between CagA (p=0.288) and VagA sl/s2 (p=0.825) tests when considering different age groups, the PCR product for 16sRNA showing difference in nine cases from all positive patients at line 446 bp, that equal to (22.5%) have deferent strain of H. Pylori and the PCR product for CagA showing difference in seven cases from all positive patients at line 599 bp that means 30.4% suffer from sever H. Pylori conclusion : One of fourth of H. Pylori infection have a chance to sever or chronic disease through presence of, CagA, and VagA sl/s2 gene in strain and female’s patients has more risk to suffer from sever H. Pylori infection through change in CagA gene.