EVALUATION OF ANTIBACTERIAL EFFICACY OF LEMON GRASS OIL, TEA TREE OIL, EUCALYPTUS OIL AND LEMON JUICE, AS A HAND SANITIZER
Keywords:
Hand sanitizer; Antibacterial; Plant extracts; Lemon juice; Eucalyptus oil,Lemon grass oil, tea tree oil.Abstract
Many studies have shown that understaffing and increased workload is risk factor for health care-associated epidemics and the goal of hand hygiene is to obtain sufficient reduction of microbial counts on the skin to prevent cross-transmission of pathogens among patients. There are also studies showing herbal hand sanitizers having significant bacteriostatic effect on hands.
The study will be undertaken to assess the antibacterial efficacy of lemon juice and three different natural oils. Three groups consisting of health workers (group I, group II, group III and Group IV) were included in the study. Sterile cotton swab sticks were used to take swabs from both hands and swabs were inoculated on the part of the agar plates marked pre application on to Cystein lactose electrolyte deficient agar plates. Lemon juice and natural oils
(Eucalyptus, Tea tree oil and Lemon grass) was asked to rub the thoroughly on the palms.
ater after 1 min, swabs were collected again from the palms and inoculation was done on the respective agar plates, in the part marked post. This procedure was repeated for six consecutive days. Lemon juice was slightly better than lemon grass oil, tea tree oil and eucalyptus oil in eradicating microbes from the hands of health care workers and also lemon grass oil showed better antibacterial efficacy than eucalyptus oil and teatree oil. Conclusion: The household herbal oils and fresh extracts can be used in preventing spreading of infections,namely by its use as an antiseptic for topical and for hand cleaning.
Downloads
References
Mondal S, Kolhapure SA. Evaluation of the antimicrobial efficacy and safety of pure
hands herbal hand sanitizer in hand hygiene and on innaminate objects. The antiseptic
;101(2):55-57.
Warnke PH, Alexander JS, Sherry E, Wiltfang J, Podschum R. The ongoing battle against
multi resistant strains: in vitro inhibition of hospital acquired MRSA, VRE, Pseudomonas,
ESBL, E. Coli and Klebsiella species in the presence of plant derived antiseptic oils. J
Craniomaxillofac Surg. 2013 Jun;41(4):321-6
Nada Khazal Kadhim Hindi., Zainab Adil Ghani Chabuck., Antimicrobial Activity of
Different Aqueous Lemon Extracts. J App Pharm Sci, 2013; 3(6): 074-078.
Wani SN, Bhalerao AK, Vikram P, Zanje R. Formulation and evaluation
of herbal hand sanitizer. Int J PharmTech Res.2013;5(1):40-43.
Jafari B, Ebadi A, Aghdam BM, Z Hassanzade Z. Antibacterial Activities
of Lemon Grass Methanol Extract and Essence on Pathogenic Bacteria. American-Eurasian J
Agric. & Environ. Sci. 2012; 12(8): 1042-1046.
Bachir RG, Benali M. Antibacterial activity of the essential oils from the leaves of
Eucalyptus globulus against Escherichia coli and Staphylococcus aureus. Asian Pac J Trop
Biomed. 2012 Sep;2(9):739-42.
Naik MI, Fomda BA, Jaykumar E, Bhat JA. Antibacterial activity of lemongrass
(Cymbopogon citratus) oil against some selected pathogenic bacterias. Asian Pac J Trop Med
;3(7):535-538.
Maruti J. Dhanavade, Chidamber B. Jalkute, Jai S. Ghosh and Kailash D. Sonawane. Study
Antimicrobial Activity of Lemon (Citrus lemon L.) Peel Extract. Br. J. Pharmacol. Toxicol.
; 2(3): 119-122.
Bessonneau V, Clément M, Thomas O. Can Intensive Use of Alcohol- Based Hand Rubs
Lead to Passive Alcoholization?. Int J Environ Res Public Health. 2010; 7(8): 3038–3050.
Mulyaningsih S, Sporer F, Reichling J, Wink M. Antibacterial activity of essential oils
from Eucalyptus and of selected components against multidrugresistant bacterial pathogens.
Pharm Biol. 2011 Sep;49(9):893-899.
Trivedi NA, Hotchandani SC. A study of the antimicrobial activity of oil of Eucalyptus.
Indian J Pharmacol2004;36(2):93-95.
Plant J, Stephens B. Evaluation of antibacterial activity of a sizable set of essential oils.
Med Aromat Plants. 2015;4(2)1-5.
Soares MO, Vinha AF, Barreira SVP, Coutinho F, Aires-Gonçalves S, Oliveira MBPP, et
al. Cymbopogon citratus EO antimicrobial activity against multi-drug resistant Grampositive
strains and non-albicans- Candida species.
www.formatex.info/microbiology4/vol 2/1081-1086.pdf.
Dimić GR, Kocić-Tanackova SD, Jovanovb OO , Cvetković DD, Markova SL,
Velićanskia AS. Antibacterial activity of lemon, caraway and basil extracts on listeria spp.
APTEFF 2012; 43: 239-246.
Olson W, Vesley D, Bode M, Dubbel P, Bauer T. Hard Surface Cleaning Performance of
Six Alternative Household Cleaners Under Laboratory Conditions. J Environ Health
:56(6):27-32.
Mcdonnell G & Russell AD. Antiseptics and Disinfectants: Activity, Action, and
Resistance. Clin.Microbiol.Rev.1999;12(1):147- 179
Downloads
Published
Issue
Section
License
You are free to:
- Share — copy and redistribute the material in any medium or format for any purpose, even commercially.
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
- The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit , provide a link to the license, and indicate if changes were made . You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Notices:
You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation .
No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.