Silver nanoparticles (AgNPs) were synthesized from silver nitrate, and sodium borohydride and the structures were characterized using ultraviolet-visible. Scanning electron microscopy was also used to measure the dimensions of silver nanoparticles. This cross-sectional study was performed on 250 clinical specimens collected from patients hospitalized/or attended to four major hospitals in Najaf city from October 2020 to February 2021. K. pneumoniae isolates identified by standard biochemical tests and confirmed by the VITEK-2 system that Klebsiella isolates (38 isolates) belong to Two species: K. pneumoniae (37 isolates), K. oxytoca (1 isolate . K. pneumoniae is isolated more frequently from urine, Wound, Burn, Sputum, Stool, Body fluids and Blood samples.