Nanoparticles (NPs) of Metals like Gold (Au), Silver (Ag) and Zinc (Zn) are getting much more attention due to their non-Immunogenitic effect, Bio-sensing and Drug delivery in medicine and industry. The advantages of such NPs are of wide range as they are chemically stable and nonreactive to Oxygen. Due to wet chemical synthesis and Bio-molecular functioning, NPs performed very vital role in drug delivery, biosensors and Photo-thermal therapy. In this study Silver Nanoparticles AgNps were used to observe the effects of Ag0 Nps on Gram Negative Resistant Bacteria.