Background: Epilepsy is the fourth most frequent neurological disorder worldwide. Epilepsy can be diagnosed if someone has two or more spontaneous seizures. Subtypes M1 and M3 of the receptor are affected by pilocarpine, with the M3 receptor having the ability to activate phospholipase C. In this way, the second messenger's inositol triphosphate, diacylglycerol, calcium, and protein kinase are produced. Because of this, M3 cholinergic agonists cause calcium to be upregulated, leading to smooth muscle contraction