ISSN 2409-5834

Molecular Study of Extended Spectrum Β-Lactamases Genes (Blashv, Blactxm) in Klebsiella Pneumoniae Isolated from Patients Infected with Pneumonia

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Angham jasim Mohammed Ali ,Miaad K. Alkhudhairy ,Dhabaa D. Tobal


This study aimed for isolation and identification of Klebsiella pneumoniae from clinical samples and detection the prevalence of some extended spectrum β-lactamases (ESBLs) genes (blaSHV, blaCTX-M) in K. pneumoniae Samples collected were clinical respiratory tract infections. A total of 210 sputum specimens during the period from December 2018 to February. Growth on blood agar, MacConkey agar was identified by cultural, morphological and biochemical tests which revealed that: 62∕ 210 (51.66%) gave positive growth for K. pneumoniae. All the 62 isolates of K. pneumoniae were screened for their antibiotic resistance against 21 antibiotics of different classes using disk diffusion method. The results showed that all the tested isolates were resistant to at least 6 antibiotics of which they were tested, hence the isolates were considered to be multidrug resistant. The results showed that all of the isolates were found to be resistant to β-lactam antibiotic (amoxicillin-clavulanate acid) Disk approximation test and not production of ESBLs by these isolates. All the 62 isolates were submitted to molecular detection of some ESBLsgenes (blaSHV, blaCTX-M) using PCR technique. The results showed that only 8(12.90%) isolates were carried blaCTX-M gene and 7(11.29%) isolates were carried blaSHV gene.

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