The current study was conducted at biology department in faculty of science / university of Kufa for molecular identification to identifiy variation between white vinca (pure variety ) and local vinca variety (pink) at both molecular level using RAPD, ISSR and SCoT markers and examining antibacterial activity of leaf methanolic, ethanolic crude extracts and phenolic crude against E.coli, Proteus, Enterococcus , K. pneumonia, S. aureus, Salmonella, Acinetobacter ,P. aeruginosa and Enterobacter.