Copper oxide (CuO) nanoparticles have attracted great interest due to their applications in many fields. CuO nanoparticles were synthesized in a hybrid way using two preparation techniques. The first is pulsed laser ablation (PLA) to remove the CuO metal target immersed in water. The ablation was carried out by an Nd: YAG laser with a power of 100 mJ at a wavelength of 1064 nm. The second process was cold plasma, where the resulting nanoparticles were placed for 5 minutes under the influence of cold plasma and at a flow rate of argon gas 3 L/min to increase the effectiveness of the nanoparticles