Vitex negundo L. belongs to the family verbenaceae. it has been reported to possess Anti-inflammatory, analgesic and anti-rheumatic activity, Antioxidant activity, Enzyme inhibitory activity,Antimicrobial Antinociceptive activity Anticonvulsant Antifungal activity Anti-tumor and anticancer activity Antidiabetic activity: Laxative. Hepatoprotective activity The present study designed to evaluate the genotoxicy of ethyl acetate fraction of vitex negundo , extract administered orally, in two different doses [100mg/kg and 200mg/kg] on both bone marrow and spleen cells in mice for seven successive days, and comparing their effects with methotrexate (positive control) and dimethylsulfoxide [DMSO](negative control).