Background-Many studies have shown that understaffing and increased workload is risk factor for health care-associated epidemics and the goal of hand hygiene is to obtain sufficient reduction of microbial counts on the skin to prevent cross-transmission of pathogens among patients. There are also studies showing herbal hand sanitizers having significant bacteriostatic effect on hands. The study will be undertaken to assess the antibacterial efficacy of lemon juice and three different natural oils.