At the present time, the phenomenon of antibiotic resistance has increased by different species of bacteria. In this way, particularly in the situations of metal nanoparticles (MNPs) fabrication and MNPs surface modification, the emergence of nanotechnology as a new weapon has drawn increased attention. Currently, the safe way to manufacture nanoscales is at the lowest possible cost and the least harm to the environment of IO NPs with novel shape through Ultrasound-Assisted. Ultraviolet–visible spectrophotometer (uv-Vis), energy dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM), atomic force microscopic (AFM), X-ray diffraction (XRD). these techniques were applied for physical characterization. well diffusion assay and minimum inhibitory concentration (MIC), were evaluated against gram negative (P.aeruginosa , Klebsiella spp) and gram positive (S. aureus , S. pyogenes) .Where the activity of iron nanoparticles prepared by a physical method showed a distinct activity against selected cancer cells. IO NPs with an average diameter size of 30nm.