ISSN 2409-5834

Detection of VIM And IMP Metallo- Beta- Lactamase Genes in Carbapenem Resistant Pseudomonas Aeruginosa Isolated from Different Clinical Infection in AL- Najaf Province

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Enas Jalil Baqer Almayali ,Abbas Shaker AL-muhana


Pseudomonas aeruginosa is an opportunistic human pathogen cause most health care-associated infections and it is considered a paradigm of antibiotic resistance development. Carbapenem resistant P. aeruginosa has emerged as an important cause of infection in different hospitals worldwide. Present study aimed to investigate the prevalence of multi drug resistant P. aeruginosa and to determine frequencies of the main Metallo-β-lactamases (MBLs) genes in isolates collected from Najaf hospitals. The results were as follows: 80 isolates of P. aeruginosa out of 230 isolates were Gram-negative bacteria and non-lactose fermenter after growing on MacConkey agar. Forty –eight isolates were resistant to the carbapenem class after conducting a sensitivity test to eighty isolates of Pseudomonas aeruginosa. The isolates were tested for susceptibility to 14 antibiotic by the Kirby-Bauer disk diffusion method to P. aeruginosa . Overall, the resistant rates for penicillin antibiotics including piperacillin were (83.75%), piperacillin/tazobactam (85 %) , ceftazidime (68.75 %) , cefepime (76.25%). aztreonam (37.5%) , imipenem (60%) and meropenem (55%) , tobramycin (52.5%), gentamicin(55%), amikacin( 62.5%). ciprofloxacin (56.25%) , levofloxacin (63.75%), norfloxacin (61.25%) and moxifloxacin (75%). As for the molecular study for the detection of Metallo-beta-lactamase genes of Pseudomonas aeruginosa bacteria with carbapenem resistant (48 isolate ), it was as follows ( bla-VIM , bla-IMP) with the following percentages (10.4%), (22.9%) respectively.

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