One hundred children with a diarrheal infection and eighteen healthy children between 4-10 years old participated in the study. Between July 2022 and October 2022, kids from the Baghdad and Basrah provinces visited clinics for diarrhea treatment. According to the data, a total of 73 clinical samples tested negative for S. typhi, whereas 27 samples tested positive for other bacteria. When used VITEK-2 system. Only 18 (66.7%) of the 27 stool isolates were found to be associated to S. typhi. At the end of a PCR reaction, billions of copies of a certain sequence will have been produced thanks to the ability of DNA polymerase to synthesize a new strand of DNA complementary to a provided template strand (Amplicon).