EVALUATING THE DIAGNOSTIC EFFICACY OF RT-PCR AND IMMUNOHISTOCHEMISTRY FOR PESTE DES PETITS RUMINANTS IN GOATS: INSIGHTS INTO AGE-RELATED SUSCEPTIBILITY IN ENDEMIC REGIONS OF PAKISTAN
DOI:
https://doi.org/10.48047/HM.10.2.2024.2058-2070Keywords:
Peste des Petits Ruminants (PPR), RT-PCR, Immunohistochemistry,, Diagnostic efficacy,, Age susceptibility,Abstract
Peste des Petits Ruminants (PPR) is a highly contagious viral disease affecting small ruminants, especially in endemic areas such as South Asia. This study evaluated the diagnostic efficiency of Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and immunohistochemistry (IHC) for detecting PPR and examined age-related susceptibility in goat populations. Sixty goats, including symptomatic and deceased specimens, were tested using RT-PCR and IHC to confirm infection and illustrate viral localization. Statistical analyses, including chi-square tests, assessed the associations between age and diagnostic outcomes. The findings revealed that RT-PCR and IHC exhibited high diagnostic accuracy, with 80% sensitivity and 100% specificity. PPR prevalence was 70% in the sampled population, with younger goats (under 12 months) showing a significantly higher infection rate, underscoring age as a critical risk factor. These results emphasize the reliability of RT-PCR and IHC in PPR diagnosis and support for implementing age-specific intervention strategies in resource-constrained endemic regions.
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Copyright (c) 2025 Muhammad Umair, Muhammad Tauseef Ahmad, Muhammad Tahir Sarfraz khan, Esha Mustafa, Rahmeen Ajaz, Mirza Muhammad Arslan Azam, Maryam Iqbal, Shahzada Khurram Adrian Shah (Author)
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