SYNTHESIS OF IRON OXIDE MAGNETIC NANOPARTICLES CONJUGATED WITH METHOTREXATE AND ESTIMATION OF ITS ANTIBACTERIAL AND ANTICANCER ACTIVITY

Authors

  • Azka Ilyas Department of Life Sciences, University of Management and Technology, Lahore, Pakistan Author
  • Maryam Akmal Department of Life Sciences, University of Management and Technology, Lahore, Pakistan Author
  • Hassan Jan Department of Life Sciences, University of Management and Technology, Lahore, Pakistan Author
  • Awais Saleem Department of Life Sciences, University of Management and Technology, Lahore, Pakistan Author
  • Hafsa Naeem Department of Life Sciences, University of Management and Technology, Lahore, Pakistan Author
  • Anum Farid Department of Agriculture Engineering and Food science, Shandong University of Technology, China Author
  • Komal Hassan Department of Life Sciences, University of Management and Technology, Lahore, Pakistan Author
  • Aleena Ghaffar Department of Life Sciences, University of Management and Technology, Lahore, Pakistan Author
  • Zomah Malik Department of Life Sciences, University of Management and Technology, Lahore, Pakistan Author
  • Maheen Latif Department of Life Sciences, University of Management and Technology, Lahore, Pakistan Author
  • Hannan Mirza Department of Life Sciences, University of Management and Technology, Lahore, Pakistan Author
  • Mehreen Fatima Department of Life Sciences, University of Management and Technology, Lahore, Pakistan Author
  • Ayesha Ameen Department of Agriculture Engineering and Food science, Shandong University of Technology, China Author

DOI:

https://doi.org/10.48047/HM.10.2.2024.1899-1907

Abstract

Cancer treatment faces significant challenges due to the non-specific toxicity and limited bioavailability of chemotherapeutic agents. This study investigates the use of Iron oxide nanoparticles (Mg  NPs) conjugated with methotrexate to enhance its anticancer efficacy against leukemia cells. Mg  NPs were synthesized via a chemical reduction method and characterized using FTIR. Methotrexate was conjugated to the nanoparticles through carbodiimide coupling, and cytotoxicity was assessed using the MTT assay on leukemia cells. The Mg -Methotrexate conjugates demonstrated significantly enhanced cytotoxicity compared to free cytarabine, reducing cell viability more effectively in leukemia cells. The antimicrobial properties of the nanocomposite also insured the reduction of the microbial growth in the presence of the nanocomposite. The conjugation improved drug stability, cellular uptake, and sustained release, supporting its potential application in targeted cancer therapy.

Downloads

Download data is not yet available.

References

Ferrari, M. (2005). Cancer nanotechnology: opportunities and challenges. Nature Reviews Cancer, 5(3), 161-171.

Peer, D., Karp, J. M., Hong, S., Farokhzad, O. C., Margalit, R., & Langer, R. (2007). Nanocarriers as an emerging platform for cancer therapy. Nature Nanotechnology, 2(12), 751-760.

Nie, S., Xing, Y., Kim, G. J., & Simons, J. W. (2007). Nanotechnology applications in cancer. Annual Review of Biomedical Engineering, 9, 257-288.

Davis, M. E., Chen, Z. G., & Shin, D. M. (2008). Nanoparticle therapeutics: an emerging treatment modality for cancer. Nature Reviews Drug Discovery, 7(9), 771-782.

Gupta, A. K., & Gupta, M. (2005). Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials, 26(18), 3995-4021.

Sun, C., Lee, J. S. H., & Zhang, M. (2008). Magnetic nanoparticles in MR imaging and drug delivery. Advanced Drug Delivery Reviews, 60(11), 1252-1265.

Mahmoudi, M., Sant, S., Wang, B., Laurent, S., & Sen, T. (2011). Superparamagnetic iron oxide nanoparticles (SPIONs): Development, surface modification and applications in chemotherapy. Advanced Drug Delivery Reviews, 63(1-2), 24-46.

Pankhurst, Q. A., Connolly, J., Jones, S. K., & Dobson, J. (2003). Applications of magnetic nanoparticles in biomedicine. Journal of Physics D: Applied Physics, 36(13), R167.

Laurent, S., Forge, D., Port, M., Roch, A., Robic, C., Vander Elst, L., & Muller, R. N. (2008). Magnetic iron oxide nanoparticles: Synthesis, stabilization, vectorization, physicochemical characterizations, and biological applications. Chemical Reviews, 108(6), 2064-2110.

Ross, J. F., Wang, H., Behm, F. G., Mathew, P., Wu, M., Booth, R., & Ratnam, M. (2004). Folate receptor type beta is a neutrophilic lineage marker and is differentially expressed in myeloid leukemia. Cancer, 100(5), 1109-1116.

Zhang, X., Xu, X., Lam, R., Giljohann, D., Ho, D., & Mirkin, C. A. (2020). Strategy for increasing drug solubility and efficacy through covalent attachment to polyvalent DNA-nanoparticle conjugates. ACS Nano, 4(8), 4836-4842.

Singh, A., Jain, S., & Sahoo, S. K. (2020). Magnetic nanoparticles for amalgamation of magnetic hyperthermia and chemotherapy: An approach towards enhanced attenuation of tumor. Materials Science and Engineering: C, 110, 110695.

Zheng, K., Wang, P., Lou, X., Zhou, Z., Zhou, L., Hu, Y., ... & Gao, X. (2024). A review of airborne micro-and nano-plastics: Sampling methods, analytical techniques, and exposure risks. Environmental Pollution, 363, 125074.

Downloads

Published

2024-07-01

Issue

Vol. 10 No. 2 (2024): 10 (2) 2024

Section

RESEARCH ARTICLE

License

Copyright (c) 2024 Amna Yaqoob, Asma Irshad, Hassan Jan, Awais Saleem, Hafsa Naeem, Anum Farid, Komal Hassan, Aleena Ghaffar, Zomah Malik, Maheen Latif, Hannan Mirza, Mehreen Fatima, Sumaira Bibi, Ayesha Ameen, Esha Imran, Ayesha Malik (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

You are free to:

  1. Share — copy and redistribute the material in any medium or format for any purpose, even commercially.
  2. Adapt — remix, transform, and build upon the material for any purpose, even commercially.
  3. The licensor cannot revoke these freedoms as long as you follow the license terms.

Under the following terms:

  1. Attribution — You must give appropriate credit , provide a link to the license, and indicate if changes were made . You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  2. No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

Notices:

You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation .

No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.

How to Cite

Ilyas, A., Akmal, M. ., Jan, H., Saleem, A., Naeem, H., Farid, A., Hassan, K. ., Ghaffar, A. ., Malik, Z. ., Latif, M. ., Mirza, H. ., Fatima, M., & Ameen, A. . (2024). SYNTHESIS OF IRON OXIDE MAGNETIC NANOPARTICLES CONJUGATED WITH METHOTREXATE AND ESTIMATION OF ITS ANTIBACTERIAL AND ANTICANCER ACTIVITY. History of Medicine, 10(2), 1899-1907. https://doi.org/10.48047/HM.10.2.2024.1899-1907