Fabrication of Curcumin-Conjugated CuO Nanoparticles and Assessment of Their Antibacterial and Anticancer Properties
DOI:
https://doi.org/10.48047/HM.10.2.2024.1888-1898Keywords:
Nanoparticles, Curcumin, MTT assay, Anticancer agent, Drug delivery agentAbstract
Nanobiotechnology is a rapidly developing sector influencing every field of study, particularly therapeutic index, and drug delivery. The core of nanobiotechnology is nanoparticles, because of their incredibly tiny size and large surface area, nanoparticles are capable of bringing about treatments and cures for illnesses that were previously not achievable. In this study, curcumin copper oxide Nanoparticles were synthesized by using the precipitation method, producing a high yield of nanoparticles within less time and unexpansive chemicals that were readily available. 0.02 mol of copper acetate and 0.01 mol of curcumin were used in this research. Initially, a neon yellow color was observed which was changed into brownish black color after 1 hour stirring at 80°C and 500 rpm. UV analysis was done which gave a peak at 370nm. FTIR analysis confirmed the conjugation of Curcumin with CuO NPs and also showed phenolic, ketonic, methyl, ethyl, and Carboxyl groups were present. Agar well diffusion and culture-based antibacterial activity was performed which gave positive results against gram-negative bacteria E.coli and gram-positive bacteria Bacillus subtilis. MTT assay was performed for anticancer activity of curcumin-conjugated Copper oxide nanoparticles. Hela Cells and MTT colored reagent were used which give purple color, the intensity of color shows the cell death. The Cur-CuO NPs show 26% Cell death within 2 hours. In the future, it can be used as an anticancer drug or can also be used as a drug delivery agent.
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