Evaluation of DNA damage by insecticides in workers of Apis mellifera by comet assay technique
DOI:
https://doi.org/10.48047/HM.10.2.2024.1879-1887Keywords:
Comet assay, DNA damage, insecticide, Gene toxicity, Tail length, Head lengthAbstract
The purpose of the research work was to study the effects of insecticides used in fields, on adult A. mellifera by determining the DNA damage to the eukaryotic cell’s nucleus. Forager worker bees of A. mellifera species samples (bees exposed to insecticide/Pesticide in field) were collected from the entrance of the bee hives in district Nankana Sahib, Sialkot, Hafiz Abad, Mandi Bhauddin, Chiniot, Gujranwala, Narowaal, Sragodha, Khishab and Kasure. Hemolymph of these honeybee’s samples was ejected by inserting capillary tube between 4th and 5th abdominal segments. Layering of slides was performed using 1% NMP agarose. The single cell gel electrophoresis or comet assay was used to measure damaged DNA in every hemocytes through Epifluorescent microscope. Three parameters i-e Tail Length, Tail DNA and Olive Tail Movement were used to assess the DNA damage caused by insecticides/Pesticides. The results indicated that overall maximum DNA damage was found in honeybees collected from district Khushab. It showed maximum length of comet head (29.05µm), length of tail (26.01µm), length of comet (55.15µm) and tail movement (17.99µm). The minimum genotoxicity was recorded in bees from Nankana sahib area as it had maximum head DNA length (72.49µm). Olive tail movement from Narowal (7.32µm). It can be concluded that indiscriminate use of insecticides or pesticides in agricultural fields or near honey bees apiaries caused not only the DNA damage to them but also affects the bee’s orientation and other physiological behavior. This in turn impact the strength of the bee colony and honey yield.
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Copyright (c) 2024 Samina Qamar, Fareeha Ambreen, Muhammad Asif Latif, Sohil Ahmad (Author); Hazrat Usman Sherani (Translator); Huda Sarwar, Arshia Tariq, Tania Nisar, Irsa Hafeez, Muhammad Qasim Raza (Author)
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