A Study to evaluate the antimicrobial susceptibility pattern of isolated strains of Klebsiella pneumoniae
DOI:
https://doi.org/10.48047/HM.10.2.2024.1781-1800Keywords:
Klebsiella pneumonia, Prevalence, Molecular Detection, Antimicrobial Susceptibility, Food-Borne InfectionsAbstract
Klebsiella pneumoniae is a deadly pathogen that can infect humans and livestock. K. pneumoniae causes an infection that is difficult to treat due to its ubiquitous presence and significant drug resistance. The present study was conducted to determine the prevalence of Klebsiella pneumoniae from raw bovine meat samples along with antibiotic resistance profiling of isolates. Isolation and identification of Klebsiella pneumoniae was done according to standard microbiological techniques and biochemical reactions respectively. The isolated strains were then subjected to the Kirby-Bauer disk diffusion method to detect antibiotic susceptibility. The results showed that out 100, 24(24%) were found positive for K. pneumoniae by biochemical and microscopic testing. The prevalence was higher in fresh beef (30%) samples than frozen samples (18%).The antimicrobial susceptibility test showed the maximum resistance was found against Ceftriaxone (54.17%), Ceftazidime (54.17%) and Cefotaxime (54.17%) while meropenem and imipenem showed maximum sensitivity for K.pneumoniae. The prevalence of multidrug-resistant isolates was 45.83%. Molecular detection of ESBL genes showed, the prevalence of blaCTX-M36.36%, blaOXA 27.27%, and blaTEM 18.18% while none of the samples was detected positive for blaNDM gene. This study indicates that the presence of K. pneumoniae is quite obvious in both fresh and frozen beef with multiple drug-resistance abilities. Carbapenem drugs are still the option to treat such food-borne infections.
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Al-Agha, A. E., Kafi, S. E., Aldeen, A. M. Z., & Khadwardi, R. H. (2017). Flash glucose monitoring system may benefit children and adolescents with type 1 diabetes during fasting at Ramadan. Saudi medical journal, 38(4), 366.
Ayati, A., Hosseini, K., Hadizadeh, A., Jalali, A., Lotfi‐Tokaldany, M., Milan, N., Bagheri, J., & Ahmadi Tafti, S. H. (2022). Surgical coronary revascularization in patients with COVID‐19; complications and outcomes: A retrospective cohort study. Health Science Reports, 5(5), e751.
Benie, C., Nathalie, G., Adjéhi, D., Solange, A., Ferniquekonan, K., & Desire, K. (2017). Prevalence and antibiotic resistance of Pseudomonas aeruginosa isolated from bovine meat, fresh fish and smoked fish. Archives of clinical microbiology, 8(3), 1-9.
Busani, T., Devine, R., Yu, X., & Seo, H.-W. (2006). Electrical and physical properties of room temperature deposited, mixed TiO2∕ SiO2 oxides. Journal of Vacuum Science & Technology A, 24(2), 369-374.
Cao, Y., Wang, J., Jian, F., Xiao, T., Song, W., Yisimayi, A., Huang, W., Li, Q., Wang, P., & An, R. (2022). Omicron escapes the majority of existing SARS-CoV-2 neutralizing antibodies. Nature, 602(7898), 657-663.
Carroll, C., Patterson, M., Wood, S., Booth, A., Rick, J., & Balain, S. (2007). A conceptual framework for
implementation fidelity. Implementation science, 2, 1-9.
Dong, N., Li, R., & Lai, Y. (2022). Klebsiella pneumoniae: Antimicrobial resistance, virulence and
therapeutic strategies. In (Vol. 12, pp. 1108817): Frontiers Media SA.
De Oliveira, D. M., Forde, B. M., Kidd, T. J., Harris, P. N., Schembri, M. A., Beatson, S. A., ... & Walker,
M. J. (2020). Antimicrobial resistance in ESKAPE pathogens. Clinical microbiology reviews, 33(3), 10-
El Aila, N. A., Tency, I., Claeys, G., Saerens, B., Cools, P., Verstraelen, H., Temmerman, M., Verhelst, R.,
& Vaneechoutte, M. (2010). Comparison of different sampling techniques and of different culture
methods for detection of group B streptococcus carriage in pregnant women. BMC infectious diseases,
, 1-8.
Ewers, M., Brendel, M., Rizk-Jackson, A., Rominger, A., Bartenstein, P., Schuff, N., Weiner, M. W., &
Initiative, A. s. D. N. (2014). Reduced FDG-PET brain metabolism and executive function predict clinical
progression in elderly healthy subjects. NeuroImage: Clinical, 4, 45-52.
Gelbíčová, T., Baráková, A., Florianová, M., Jamborová, I., Zelendová, M., Pospíšilová, L., Koláčková, I.,
& Karpíšková, R. (2019). Dissemination and comparison of genetic determinants of mcr-mediated
colistin resistance in Enterobacteriaceae via retailed raw meat products. Frontiers in Microbiology, 10,
Gopalakrishnan, V., Spencer, C. N., Nezi, L., Reuben, A., Andrews, M. C., Karpinets, T. V., Prieto, P.,
Vicente, D., Hoffman, K., & Wei, S. C. (2018). Gut microbiome modulates response to anti–PD-1
immunotherapy in melanoma patients. Science, 359(6371), 97-103.
Guo, J. Y., & White, E. (2016). Autophagy, metabolism, and cancer. Cold Spring Harbor symposia on
quantitative biology,
Huang, C., Wang, Y., Li, X., Ren, L., Zhao, J., Hu, Y., Zhang, L., Fan, G., Xu, J., & Gu, X. (2020). Clinical
features of patients infected with 2019 novel coronavirus in Wuhan, China. The lancet, 395(10223),
-506.
Junaid, M., Liu, S., Liao, H., Liu, X., Wu, Y., & Wang, J. (2022). Wastewater plastisphere enhances
antibiotic resistant elements, bacterial pathogens, and toxicological impacts in the environment.
Science of the Total Environment, 841, 156805.
Khan, M., Khan, H., Khan, S., & Nawaz, M. (2020). Epidemiological and clinical characteristics of
coronavirus disease (COVID-19) cases at a screening clinic during the early outbreak period: a singlecentre study. Journal of medical microbiology, 69(8), 1114-1123.
Kislaya, I., Gonçalves, P., Gómez, V., Gaio, V., Roquette, R., Barreto, M., Sousa-Uva, M., Torres, A. R.,
Santos, J., & Matos, R. (2022). SARS-CoV-2 seroprevalence in Portugal following the third epidemic
wave: results of the second National Serological Survey (ISN2COVID-19). Infectious Diseases, 54(6),
-424.
Krause, K. M., Serio, A. W., Kane, T. R., & Connolly, L. E. (2016). Aminoglycosides: an overview. Cold
Spring Harbor perspectives in medicine, 6(6), a027029.
Lee, Y., Kim, M., Han, J., Yeom, K. H., Lee, S., Baek, S. H., & Kim, V. N. (2004). MicroRNA genes are
transcribed by RNA polymerase II. The EMBO journal, 23(20), 4051-4060.
Marr, C. M., & Russo, T. A. (2019). Hypervirulent Klebsiella pneumoniae: a new public health threat.
Expert review of anti-infective therapy, 17(2), 71-73.
Martin, R. M., & Bachman, M. A. (2018). Colonization, infection, and the accessory genome of Klebsiella pneumoniae. Frontiers in cellular and infection microbiology, 8, 4.
Messele, Y. E., Abdi, R. D., Yalew, S. T., Tegegne, D. T., Emeru, B. A., & Werid, G. M. (2017). Molecular
determination of antimicrobial resistance in Escherichia coli isolated from raw meat in Addis Ababa
and Bishoftu, Ethiopia. Annals of clinical microbiology and antimicrobials, 16, 1-9.
Montso, K. P., Dlamini, S. B., Kumar, A., & Ateba, C. N. (2019). Antimicrobial Resistance Factors of
Extended‐Spectrum Beta‐Lactamases Producing Escherichia coli and Klebsiella pneumoniae Isolated
from Cattle Farms and Raw Beef in North‐West Province, South Africa. BioMed research international,
(1), 4318306.
Muñoz, J. P., Ivanova, S., Sánchez‐Wandelmer, J., Martínez‐Cristóbal, P., Noguera, E., Sancho, A., Díaz‐
Ramos, A., Hernández‐Alvarez, M. I., Sebastián, D., & Mauvezin, C. (2013). Mfn2 modulates the UPR
and mitochondrial function via repression of PERK. The EMBO journal, 32(17), 2348-2361.
Nandi, A., Yan, L.-J., Jana, C. K., & Das, N. (2019). Role of catalase in oxidative stress‐and age‐associated
degenerative diseases. Oxidative medicine and cellular longevity, 2019(1), 9613090.
Navon-Venezia, S., Kondratyeva, K., & Carattoli, A. (2017). Klebsiella pneumoniae: a major worldwide
source and shuttle for antibiotic resistance. FEMS microbiology reviews, 41(3), 252-275.
Nayak, A. H., Kapote, D. S., Fonseca, M., Chavan, N., Mayekar, R., Sarmalkar, M., & Bawa, A. (2020).
Impact of the coronavirus infection in pregnancy: a preliminary study of 141 patients. The Journal of
Obstetrics and Gynecology of India, 70, 256-261.
Paterson, R. R. M. (2006). Ganoderma–a therapeutic fungal biofactory. Phytochemistry, 67(18), 1985-
Pisal, D. S., & Yadav, G. D. (2021). Production of biofuel 2, 5-dimethylfuran using highly efficient singlestep selective hydrogenation of 5-hydroxymethylfurfural over novel Pd-Co/Al-Zr mixed oxide catalyst.
Fuel, 290, 119947.
Podschun, R., Pietsch, S., Höller, C., & Ullmann, U. (2001). Incidence of Klebsiella species in surface
waters and their expression of virulence factors. Applied and environmental microbiology, 67(7), 3325-
Qutub, M., Aldabbagh, Y., Mehdawi, F., Alraddadi, A., Alhomsy, M., Alnahdi, A., Fakeeh, M., Maghrabi,
A., Alwagdani, M., & Bahabri, N. (2022). Duration of viable SARS-CoV-2 shedding from respiratory tract
in different human hosts and its impact on isolation discontinuation polices revision; a narrative
review. Clinical Infection in Practice, 13, 100140.
Roca, I., Akova, M., Baquero, F., Carlet, J., Cavaleri, M., Coenen, S., Cohen, J., Findlay, D., Gyssens, I.,
& Heure, O. (2015). The global threat of antimicrobial resistance: science for intervention. New
microbes and new infections, 6, 22-29.
Rodrigues, M., Camprubí, À. C., Balaguer-Romano, R., Megía, C. J. C., Castañares, F., Ruffault, J.,
Fernandes, P. M., & de Dios, V. R. (2023). Drivers and implications of the extreme 2022 wildfire season
in Southwest Europe. Science of the Total Environment, 859, 160320.
Shon, H.-S., Choi, H. Y., Kim, J. R., Ryu, S. Y., Lee, Y.-J., Lee, M. J., Min, H. J., Lee, J., Song, Y. J., & Ki, M.
(2015). Comparison and analysis of the prevalence of hepatitis C virus infection by region in the
Republic of Korea during 2005-2012. Clinical and Molecular Hepatology, 21(3), 249.
Theocharidi, N. A., Balta, I., Houhoula, D., Tsantes, A. G., Lalliotis, G. P., Polydera, A. C., Stamatis, H., &
Halvatsiotis, P. (2022). High prevalence of Klebsiella pneumoniae in Greek meat products: Detection
of virulence and antimicrobial resistance genes by molecular techniques. Foods, 11(5), 708.
Tripathi, N., & Sapra, A. (2020). Gram staining.
Waghmode, S., Suryavanshi, M., Sharma, D., & Satpute, S. K. (2020). Planococcus species–an imminent
resource to explore biosurfactant and bioactive metabolites for industrial applications. Frontiers in
bioengineering and biotechnology, 8, 996.
Zheng, H., Wang, Z., Deng, X., Herbert, S., & Xing, B. (2013). Impacts of adding biochar on nitrogen
retention and bioavailability in agricultural soil. Geoderma, 206, 32-39.
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