Screening and biochemical characterization of beta hemolytic strains of Staphylococcus aureus from Conjuctivitis infections
DOI:
https://doi.org/10.48047/Keywords:
Conjunctivitis, Staphylococcus aureus, Mannitol salt agar, Beta Hemolysis, Antibiotic resistantAbstract
Background: Conjunctivitis is one of the most common infections observed in hospitals. Children under the age of seven are at the highest risk for diagnosis, with the most notable incidence occurring between the ages of zero and four. Objective: The present study was aimed to identify pathogenic strains of Staphylococcus aureus by biochemical tests. Methodology: Samples of eye infection were collected from Fatima Memorial Hospital, Lahore. After collection, Samples were spread on Mannitol salt agar (MSA) to get pure culture of S. aureus. After isolating pure bacterial strains, the pathogenicity of these strains was assessed using a blood agar test. The strains that exhibited beta hemolysis were selected, and their susceptibility to various antibiotics was evaluated. Results: Results showed that all used antibiotics were considerably effective against the pathogenic isolated bacteria. Five strains displayed beta hemolysis, suggesting the existence of pathogenic strains of Staphylococcus aureus, known to be associated with Conjunctivitis. Zones of inhibition (ZOI) were noted in response to various antibiotics. Conclusion: Effective and accessible treatment could enhance public health worldwide. Easily obtainable treatments may result in improved health outcomes, especially in areas where eye infections are common and access to advanced medical care is restricted. The antibiotic sensitivity test indicated that S. aureus is a multidrug-resistant pathogen, with only a small percentage of antibiotics proving effective against it.
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