PCR detection of Staphylococcus aureus isolated from sub-clinical mastitis of goat with expression immune responses genes.

Abstract

Objective: Goat sub-clinical mastitis is a multi-bacterial infection. The proper selection of a medicine for therapy depends on correctly identifying the bacterium. Via use of the 16S ribosomal RNA (16S rRNA) gene, the goal of this work is to quantify the gene expression of IL2 and TNFa while also identifying the phenotypic and genotypic traits of Staphylococcus aureus that contribute to illness. Materials and Methods: Utilizing 16S rRNA gene-specific universal primers for the bacterial polymerase chain reaction (PCR), 100 samples of goats with healthy and subclinical mastitis were analyzed. The expression levels of the staphylococcus aurus and the inflammatory cytokines interleukin (IL-2) and (TNFa) in sub-clinical mastitis and healthy animals were evaluated and compared using the primers.Results: According to the PCR, 70 (64.3%) samples had favorable outcomes. Sub-clinical mastitis goats had considerably higher TNF gene expression than healthy goats (p˂ 0.001). The relative gene expression in subclinical mastitis-affected animals of TNFa and IL2 is 6.2±3.23 and 1.02±0.311, respectively, and the relative gene expression of these cytokines is 3.012±2.12 and 3.33±0.821, respectively.