Effect of Isotiocyanate Therapy On Trophoblast Cell Culture Hyperglycemia Atmosphere In Apoptosis, Caspase-3, NO, VEGF
Keywords:Diabetes mellitus, isotiocyanate, VEGF, nitric oxide, caspase-3, apoptosis.
Diabetes mellitus (DM) is a metabolic disorder characterized by chronic hyperglycemia. It is caused by abnormalities in insulin secretion, impaired insulin work or disorders of both. One of the factors that cause hypertension is the deficiency of vasodilators, namely nitric oxide (NO). In preeclampsia conditions occur hypoxia or lack of oxygen. Vascular Endothelial Growth Factor (VEGF) is produced by the placenta and decreases when the placenta lacks oxygen. Isotiocyanate has prominent anti-oxidant, anti-inflammatory, anti-microbial, neuroprotective and cardioprotective activity. Prepared bottle containing cord solution solution from refrigerator (temperature 4 °C). Immediately after birth, the placenta is cut off and directly inserted into the cord solution. The method of isolation and culture of trophoblast cells is carried out based on modifications of the enzymatic isolation method. In the sampling of the placenta to be taken to the laboratory, a transport medium is needed to keep the trophoblast cells alive. Some media can be used as media, for example: Dispase produced by Roche, DNAse, Phospate Buffred Saline (PBS), etc. In this dissertation research, transport media uses PBS (Zivkovic, 2011). Previously the base of the 6 well culture plate was coated with a glass cover and dripped with ± 0.5-1 ml of gelatin (0.2%) and incubated for ± 30-60 minutes. It can be concluded that there is an effect of isotiocyanate therapy can affect the decrease in apoptosis and caspas 3 at dosing 4 as much as 0.8 mg / day / kg BB with an average of 6.12 and 4.12.