ISSN 2409-5834

Partial Purification And Molecular Weight Determination Of Glutathione S-Transferase Enzyme By Gel filtration Method In Patient With Beta Thalassemia Major

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Ali Rahman Nama ,Sayran Staar Saleh ,Wassan Nazhan Hussein

Abstract

The glutathione S-transferase (GST) enzyme was partially purified by using gel filtration chromatography technique, from the blood serum of a patient with beta thalassemia major, using a separation column with dimensions (2 cm in diameter and 100 cm in height), and Sephadex G-75 gel was used. Precipitation was done using ammonium sulfate, and dialysis was performed for the precipitated part in which the enzyme activity was high. Three packages for the activity of GST enzyme were obtained, and the activity of GST in the second package (B) was higher than the other packages, and from it the approximate molecular weight of the enzyme was determined, Also, standard calibration curves were used for a number of standard proteins with known molecular weights to obtain the linear equation from which the approximate molecular weight of GST was determined to be about (25882 Daltons).

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