Study of some morphological and chemical characteristics in two varieties of Medicago sativa L. plant with the effect of biofertilizer (AG) and Brassinolide
Department of Biology, Collage of Education Pure Science/ Ibn Al-Haitham, University of Baghdad, Iraq.
Department of Biology, Collage of Education Pure Science/ Ibn Al-Haitham, University of Baghdad, Iraq.
Abstract
The experiment was carried out in the field of the Botanical Garden belonging to the Department of Biology, College of Education for Pure Science/ Ibn Al-Haitham, University of Baghdad, for the growing season 2021-2022 to study the effect of biofertilizer (AG 8-1-9) in three concentrations (0, 3, 6) mg.L-1 and the Brassinolide hormone in four concentrations (0, 2, 4, 6) mg. L-1 and their interactions in some morphological and chemical characteristics of two varieties of Medicago sativa plant. The experiment was designed according to Rondomized Complete Blocks Design (R.C.B.D.) with three replicates per treatment. The means were compared using the least significant difference at the probability level (0.05), and the results showed that the effect of Brassinolide in different concentrations led to a significant increase in all studied characteristics, and the concentration of Brassinolide 4 mg.L-1 was exceeded in each of the number of branches, the number of root nodes, the content of chlorophyll a and b and the percentage of protein, and the concentration of Brassinolide 6 mg.L-1 was exceeded in the height of the plant characteristic. The effect of the biofertilizer in different concentrations led to a significant increase in all the studied characteristics were at a concentration of 3 mg.L-1, except for the height of the plant at the concentration of 6 mg.L-1. All duel interactions had a significant effect on all the studied characteristics with the superiority of the treatment (3, 4) mg.L-1 in the number of branches, the number of root nodes and the content of leaves of chlorophyll a and b, the treatment (3, 6) mg.L-1 in the percentage of protein and the treatment of (4, 6) mg.L-1 in plant height and the superiority of the Indian variety in the number of branches, the number of root nodes and the content of leaves from chlorophyll a and b and the percentage of protein, and the superiority of the local variety in plant height. Triple interactions led to a significant increase in all studied characteristics.
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