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Effect of alginate on the oocytes viability

Mudhafar Abdulhussein M
Sousse University-Tunisia Faculty of Medicine Ibn Al Jazzar Medical Education Center
Latifa Lassoud
Sousse University-Tunisia Faculty of Medicine Ibn Al Jazzar Medical Education Center
Muhammad-Baqir M-R.Fakhrildin
Jabir ibn Hayyan Medical University/Iraq

Abstract

Alginate is gotten from brown seaweed. Has been used broadly applied for several biomedical uses. The structure of alginate is similar to extracellular conditions of living tissues lest wide use in cell transplantation can be make alginate hydrogels by many cross-linking procedures. On the other hand, alginate gel can injection into the body, which permits wide applications in the pharmaceutical field. This study aims to assess the influence of alginate on oocyte viability. Use the alginate in 1% concentration around the oocytes to evaluate viability. The study investigated the possibility of using alginate gel with oocytes to clear the effect on the viability of oocytes. From a local slaughterhouse collect of the sheep ovaries and transportation to the laboratory during two-thirds of an houre. Then, aspirated the oocytes from the ovaries were by needle aspiration technique, and incubated for less than 1 hour. Prepare alginate gel from alginate powder in 1 % concentration  ( prepared through adding 1 g of alginate powder to 100 mL DW ). Then, mix with oocytes to assess the effect on viability.

Keywords: oocytes viability; alginate; Health ,

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