History

Copper oxide (CuO) nanoparticles have attracted great interest due to their applications in many fields. CuO nanoparticles were synthesized in a hybrid way using two preparation techniques. The first is pulsed laser ablation (PLA) to remove the CuO metal target immersed in water. The ablation was carried out by an Nd: YAG laser with a power of 100 mJ at a wavelength of 1064 nm. The second process was cold plasma, where the resulting nanoparticles were placed for 5 minutes under the influence of cold plasma and at a flow rate of argon gas 3 L/min to increase the effectiveness of the nanoparticles. The UV- Visible spectrum has also been characterized to investigate its optical properties. Atomic force microscopy (AFM.), Field Emission Scanning electron microscopy (FE-SEM). The results show that copper nanoparticles can be successfully prepared in an aqueous medium with a laser energy of 100 mJ at a wavelength of 1064 nm, and the concentration of CuO NPs by atomic absorption is equal to 35(µg/ml). These particles were used as an antibacterial agent against gram-positive (Staphylococcus aureus) and gram-negative bacteria (Escherichia coli) using the agar diffusion method, where its effectiveness was proven by measuring the inhibition diameter of the prepared dishes.