Medical Application of Staphylolysin Enzyme Lasa Against Pathogenic Bacteria and Fungi
Department of Biotechnology, College of Applied Science, University of Technology, Baghdad, Iraq
Department of Biotechnology, College of Applied Science, University of Technology, Baghdad, Iraq
Abstract
In the study, Pseudomonas aeruginosa was isolated and identified from 100 clinical samples, including 50 midstream urine samples from patients with urinary tract infections (UTIs), 25 burns swabs from burn patients, and 25 wound swabs from injured patients. In this study, 100 clinical samples were cultured on nutrient agar, MacConkey agar, blood agar, CCA agar, and cetrimide agar plates for the isolation and identification of P.aeruginosa. 67% (67/100) of the samples showed positive bacterial cultures, while the remaining 33% (33/100) of the samples showed no growth, Just 34.3% (23/67) of the 67 positive culture samples contained P. aeruginosa isolates, while 65.7% (44/67) contained isolates from other genera the P. aeruginosa isolates are fully resistance 23 (100%) to three of the antibiotics of interest during the current study, particularly Cefazolin, Ceftriaxone and Trimethoprim/Sulfamethoxazole. Meropenem was the most effective antimicrobial agents against P. aeruginosa isolates , was showed high percentage of sensitivity to it 20 (86.95%), followed by Ciprofloxacin 16 (69.566%) and Piperacillin /Tazobactam 4 (60.86%),other antibiotics ewe effective at different levels. The result of antimicrobial activity in MIC and MLC (MBC and MFC) exhibited by antibiotics and LasA enzyme extract against tested Acinetobacter baumannii ,Klebsiella pneumoniae, Escherichia coli Enterococcus faecalis, and Candida albicans the Minimal Inhibitory Concentrations (MICs) of all antibiotic that used during study ranged between 4 -32 µg/ml, while MIC of LasA extract 2- 32 µg/ml E.coli, was the most sensitive to LasA enzyme extract due to a low concentration of this antimicrobial (8µg/ml) was enough to kill it compared to, A.baumannii since a high concentration of LasA enzyme extract (64µg/ml) could kill it. E.coli and E. faecalis isolates showed synergistic action to the combination of LasA Enzyme extract and gentamycin , Vancomycin respectively, but the isolates of K. pneumoniae, A.baumannii and C. albicans showed antagonism action to the combination of LasA Enzyme extract and gentamycin , Fluconazole.
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