History

Dapsone (Dap), Streptomycin (STR) and Ampicillin (AMP) are β-lactum antibiotic drugs. For the simultaneous assessment of Dapsone, Streptomycin, and Ampicillin in dose form, sensitive, accurate, and straightforward (UV-Vis) spectrophotometric procedures have been established. Using spectrophotometric methods, a simultaneous equation was used to determine the medications as a mixture, Maximum drug absorbance was reported to occur at (273.5 nm), (283.5 nm), and (257) for Dap, STR, and AMP, respectively, in a solvent mixture of water and methanol in a ratio of (80% - 20%).The established method was used to choose these wavelengths for the examination of drugs in manufactured samples as well as mixture standards. The method was linear in the range of (1-100 µg / ml) for ( Dap, STR and AMP ) , with an R² of (0.9992) for Dap ,R² for STR (0.9996) and R² for AMP (0.9995) respectively in the mixture . Recovery means for the standard drugs were found to be (98.9987-101.3674) for Dap , (98.7551-101.0865) for STR and (99.6671-100.3371) for AMP . LOD was determined to be for Dapsone (0.3778), Streptomycin (2.10815), and Ampicillin (2.1968), while LOQ was determined to be for Dapsone (1.2467), Streptomycin (6.9568), and Ampicillin (2.1968). The technique was used to estimate the drug active gradient in various manufactured dosage samples. The mean percentage recovery was used to validate the method's accuracy, and it was discovered to be within acceptable bounds.