Evaluation of the influence of the vitex negundo ethyl acetate fraction on the frequency of micronuclei and the mitotic index in mice
Department of Pharmacology and Toxicology, College of pharmacy, University of Baghdad , Baghdad, Iraq,
Department of Pharmacology and Toxicology, College of pharmacy, University of Baghdad, Baghdad, Iraq
Abstract
Vitex negundo L. belongs to the family verbenaceae. it has been reported to possess Anti-inflammatory, analgesic and anti-rheumatic activity, Antioxidant activity, Enzyme inhibitory activity,Antimicrobial Antinociceptive activity Anticonvulsant Antifungal activity Anti-tumor and anticancer activity Antidiabetic activity: Laxative. Hepatoprotective activity The present study designed to evaluate the genotoxicy of ethyl acetate fraction of vitex negundo , extract administered orally, in two different doses [100mg/kg and 200mg/kg] on both bone marrow and spleen cells in mice for seven successive days, and comparing their effects with methotrexate (positive control) and dimethylsulfoxide [DMSO](negative control). The results have been showed that ethyl acetate fraction of vitex negundo at a dose 100mg/kg and 200mg/kg showed a significant increase of mitotic index in bone marrow cells and spleen cells in comparison with DMSO, meanwhile it showed a significant decrease of micronucleus appearance in bone marrow cells, in conclusion ethyl acetate fraction of vitex negundo showed protective effect on mitotic index and micronucleus appearance in bone marrow cells and spleen cells in mice.
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