History

The first method on the oxidative coupling Cefotaxime reaction with 2,4- dinitrophenyl hydrazine and Sodium hydroxide to produce a soluble Reddish- Orange dye product was devised to estimate the Cefotaxime in bulk and pharmaceutical preparation. The concentration range is between 1 and 50 g/mL. Beer's law is met by the product in terms. With a molar absorptivity of 6.6×104L.mol-1.cm-1 and a correlation coefficient of 0.9995, Sandell's sensitivity is 6.85×10¯³ g.cm-2  at  wave length 615 nm. A second technique for determining a trace amount in an aqueous solution product is called cloud point extraction (CPE), and it is based on measurements made with a UV-visible spectrophotometer at a wavelength of 610 nm. The correlation coefficient was 0.9995 and the Beer's law concentration range was 0.25–6 g/mL The molar absorptivity was 1.4×104 L.mol-1.cm-1. Pre-concentration 25, detection of limit 0.021 µg/mL. The distribution coefficient (D) was 298.97, and enrichment factor was 18.59. The last method is flow injection analysis, which is straightforward to estimate. The Beer's law concentration range was 2-150 µg / mL, Sandell's sensitivity is 0.11 µg.cm-2 at 605 nm, with a molar absorptivity of 4×103 L.mol-1.cm-1 and correlation coefficient was 0.9995, it is successfully used for drug estimation. Qualities in a straightforward situation or pharmacological formulations.