History of Medicine


An Open Access Journal

Spectrophotometric assessment of Amino drugs employing flow injection analysis and cloud point extraction techniques

Dalia M. Jamil
Department of Chemistry, College of Science, Al_Nahrain University, Baghdad, Iraq.
Nisreen K. Abood
Department of Chemistry, College of Science, Mustansyria University, Baghdad, Iraq.


The first method on the oxidative coupling Cefotaxime reaction with 2,4- dinitrophenyl hydrazine and Sodium hydroxide to produce a soluble Reddish- Orange dye product was devised to estimate the Cefotaxime in bulk and pharmaceutical preparation. The concentration range is between 1 and 50 g/mL. Beer's law is met by the product in terms. With a molar absorptivity of 6.6×104L.mol-1.cm-1 and a correlation coefficient of 0.9995, Sandell's sensitivity is 6.85×10¯³ g.cm-2  at  wave length 615 nm. A second technique for determining a trace amount in an aqueous solution product is called cloud point extraction (CPE), and it is based on measurements made with a UV-visible spectrophotometer at a wavelength of 610 nm. The correlation coefficient was 0.9995 and the Beer's law concentration range was 0.25–6 g/mL The molar absorptivity was 1.4×104 L.mol-1.cm-1. Pre-concentration 25, detection of limit 0.021 µg/mL. The distribution coefficient (D) was 298.97, and enrichment factor was 18.59. The last method is flow injection analysis, which is straightforward to estimate. The Beer's law concentration range was 2-150 µg / mL, Sandell's sensitivity is 0.11 µg.cm-2 at 605 nm, with a molar absorptivity of 4×103 L.mol-1.cm-1 and correlation coefficient was 0.9995, it is successfully used for drug estimation. Qualities in a straightforward situation or pharmacological formulations.

Keywords: Cefotaxime, Batch method, Cloud point extraction, Flow injection analysis. ,


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